滇黄芩茎叶乙醇提取物及其不同溶剂萃取部位的抗氧化和降脂活性研究  

Study on Antioxidant Activity and Lipid-lowering Effect of Ethanol Extract and Its Different Solvent Extracts from the Stem and Leaves of Scutellaria amoena

作　　者：李欣坪 王蒙蒙 王子晨 方琼莲 林玉萍 LI Xinping;WANG Mengmeng;WANG Zichen;FANG Qionglian;LIN Yuping(School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院/云南省南药可持续利用重点实验室,昆明650500

出　　处：《中国药房》2021年第2期220-225,共6页China Pharmacy

基　　金：云南省科技厅云南中医学院应用基础研究联合专项[No.2017F117(-022)];云南中医药大学大学生创新、创业训练项目(No.2019)。

摘　　要：目的:研究滇黄芩茎叶乙醇提取物及其不同溶剂萃取部位的抗氧化活性和降脂活性。方法:取滇黄芩茎叶用95%乙醇回流提取,得乙醇提取物;取上述提取物,依次用石油醚、乙酸乙酯、正丁醇萃取,回收溶剂得到不同溶剂萃取部位。以维生素C(Vc)为阳性对照,采用羟基自由基、超氧阴离子自由基、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法检测滇黄芩茎叶乙醇提取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物的体外抗氧化活性并计算半数抑制浓度(IC50)。以脂肪乳建立脂肪变性L02肝细胞模型,以非诺贝特(20μg/m L)为阳性对照,考察高、低质量浓度(100、50μg/mL)滇黄芩茎叶乙醇提取物、乙酸乙酯萃取物、正丁醇萃取物对细胞中TC、TG含量的影响。结果:对羟基自由基的清除能力强弱排序为滇黄芩茎叶正丁醇萃取物>乙酸乙酯萃取物>Vc>乙醇提取物>石油醚萃取物,IC50分别为0.15、0.17、0.35、0.75、1.17 mg/mL;对超氧阴离子自由基的清除能力强弱排序为Vc>正丁醇萃取物>乙酸乙酯萃取物>乙醇提取物>石油醚萃取物,IC50分别为0.034、0.55、0.75、3.32、3.73 mg/mL;对DPPH自由基的清除能力强弱排序为Vc>正丁醇萃取物>乙酸乙酯萃取物>乙醇提取物>石油醚萃取物,IC50分别为0.003 2、0.028、0.033、0.048、0.057 mg/mL。滇黄芩茎叶乙醇提取物、乙酸乙酯萃取物和正丁醇萃取物对脂肪变性L02肝细胞中TC、TG含量均有显著的降低作用(P<0.01),其降脂能力强弱排序为正丁醇萃取物(低浓度)≈非诺贝特>乙酸乙酯萃取物(高浓度)>乙醇提取物(高浓度)>正丁醇萃取物(高浓度)>乙酸乙酯萃取物(低浓度)>乙醇提取物(低浓度)。结论:滇黄芩茎叶乙醇提取物、石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物均有一定的抗氧化活性和降脂活性(除石油醚萃取物外),其中正丁醇和乙酸乙酯萃取物的抗氧化活性和降脂活性较OBJECTIVE:To study the antioxidant activity and lipid-lowering effect of ethanol extract and its different solvent extracts from the stems and leaves of Scutellaria amoena.METHODS:The stem and leaves of S.amoena was extracted with 95%ethanol to obtain ethanol extract,and then extracted with petroleum,ethyl acetate and n-butanol to obtain corresponding different solvent extracts.Using vitamin C(Vc)as positive control,the antioxidant activities of ethanol extract,petroleum ether extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena were determined by hydroxyl radical,superoxide anion radical and DPPH radical scavenging method,and the IC50 was calculated.Steatosis L02 hepatocyte model was established with fat emulsion.Using fenofibrate(20μg/mL)as positive control,the effects of high and low concentration(100 and 50μg/mL)ethanol extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena on the contents of TC and TG in cells were investigated.RESULTS:The order of scavenging ability to hydroxyl radicals was n-butanol extract>ethyl acetate extract>Vc>ethanol extract>petroleum ether extract;IC50 of them were 0.15,0.17,0.35,0.75,1.17 mg/mL,respectively.The order of scavenging ability to superoxide anion radical was Vc>n-butanol extract>ethyl acetate extract>ethanol extract>petroleum ether extract;IC50 of them were 0.034,0.55,0.75,3.32,3.73 mg/mL,respectively.The order of DPPH scavenging ability to DPPH radical was Vc>n-butanol extract>ethyl acetate extract>ethanol extract>petroleum ether extract;IC50 of them were 0.0032,0.028,0.033,0.048,0.057 mg/mL,respectively.The ethanol extract,ethyl acetate extract and n-butanol extract from the stems and leaves of S.amoena could significantly decrease the contents of TC and TG in steatosis L02 hepatocytes(P<0.01).The order of lipid-lowering ability was n-butanol extract(low dose)≈fenofibrate>ethyl acetate extract(high dose)>ethanol extract(high dose)>n-butanol extract(high dose)>ethyl acetate extract(low dose)>et

关 键 词：滇黄芩茎叶 乙醇提取物 抗氧化活性 降脂活性 

分 类 号：R285[医药卫生—中药学]