一株伯克霍尔德菌的鉴定及其对木质素的降解特性研究  

Identification and lignin degradation characteristics of a Burkholderia sp

作　　者：马英辉[1,2] 李利军 卢美欢[1] 仝泽方[1] 吴文朋 MA Yinghui;LI Lijun;LU Meihuan;TONG Zefang;WU Wenpeng(Microbiology Institute of Shaanxi,Xi’an Shaanxi 710043;College of Natural Resources and Environment,Key Laboratory of Plant Nutrition and Agro-environment in Northwest China,Ministry of Agriculture and Rural Affairs,Northwest A&F University,Yangling Shaanxi 712100)

机构地区：[1]陕西省微生物研究所,陕西西安710043 [2]西北农林科技大学资源环境学院,农业农村部西北植物营养与农业环境重点实验室,陕西杨陵712100

出　　处：《环境污染与防治》2021年第1期25-29,41,共6页Environmental Pollution & Control

基　　金：陕西省科学院应用基础研究基金资助项目(No.2015K-14);陕西省科技厅农业创新与公关项目(No.2016NY-197);陕西省重点产业创新链(群)项目(No.2019ZDLNY01-02-01)。

摘　　要：从造纸厂污水中筛选获得一株木质素降解细菌(记为菌株BNS),利用全自动微生物鉴定仪和16S rRNA对菌株BNS进行鉴定,优化了菌株BNS对碱性木质素的降解参数,分析菌株BNS对碱性木质素的降解机制。结果表明,菌株BNS为洋葱伯克霍尔德菌(Burkholderia.cepacia),GenBank登录号为KY681449.1,该菌株降解碱性木质素的最佳温度为30℃、最佳pH为9.0,0.5 mmol/L的Cu 2+、Fe 2+能够提高菌株BNS对碱性木质素的降解;菌株BNS对100~2000 mg/L碱性木质素的降解过程符合一级反应动力学方程,降解常数分别为0.0065~0.0273 h-1。通过降解产物分析,菌株BNS通过自身的过氧化物酶系将碱性木质素的聚合结构裂解,降解主要发生在苯环间的C—C、C—O—C等部位,最终降解产物主要为醛类和有机酸类。A lignin degradation strain was screened from the papermarking wastewater and named as BNS.The strain BNS was identified by using Biolog and 16S rRNA,the degradation parameters to alkaline lignin were optimized and the degradation mechanism was analyzed.Results showed that the strain BNS was identified as Burkholderia cepacia and the GenBank accession number was KY681449.1.The optimal temperature was 30℃,the optimal pH was 9.0 for alkaline lignin degradation and 0.5 mmol/L of copper ions and ferrous ions could enhance the degradation rate.The degradation process accords with the first order kinetics equation in the range of 100-2000 mg/L and the degradation rate constants were ranged 0.0065-0.0273 h-1.Through degradation product analysis,the strain BNS cracked the polymerization structure of alkaline lignin with own peroxidase system,and the degradation mainly occurred in C—C,C—O—C and other parts of benzene rings,and the final degradation products were mainly aldehydes and organic acids.

关 键 词：木质素 细菌 鉴定 降解特性 产物分析 

分 类 号：X172[环境科学与工程—环境科学]