3种多环芳烃对海胆细胞色素 CYP17A1 基因表达的影响  

Effects of 3 Kinds of PAHs on the Expression of CYP17A1 Genes of Strongylocentrotus intermedius

作　　者：刘帅 宿甜甜 刘长发 魏海峰[1,2,3] 赵肖依 宋雪[1,2,3] Liu Shuai;Su Tiantian;Liu Changfa;Wei Haifeng;Zhao Xiaoyi;Song Xue(Key Laboratory of Coastal Marine Environmental Science and Technology of Liaoning Province,Dalian 116023,China;School of Marine Science and Environmental Engineering,Dalian Ocean University,Dalian 116023,China;Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]辽宁省近岸海洋环境科学与技术重点实验室,大连116023 [2]大连海洋大学海洋科技与环境学院,大连116023 [3]大连海洋大学,农业农村部北方海水增养殖重点实验室,大连116023

出　　处：《生态毒理学报》2020年第5期345-351,共7页Asian Journal of Ecotoxicology

基　　金：国家海洋公益性行业科研专项(201305002);辽宁省教育厅服务地方项目(DL201804);国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金资助项目(201309,201809);国家海洋局近岸海域生态环境重点实验室基金资助项目(201013);大连海洋大学农业农村部北方海水增养殖重点实验室基金资助项目(2018-KF-21)。

摘　　要：为揭示不同种类多环芳烃(PAHs)污染物对中间球海胆(Strongylocentrotus intermedius)的生态毒理效应,研究了不同浓度的苯并(a)芘(1、5和20μg·L-1)以及2种烷基多环芳烃3-甲基菲(5、10和100μg·L-1)和2-甲基蒽(5、10和50μg·L-1)对海胆细胞色素CYP17A1基因表达的影响。结果表明,随着暴露时间的增加,不同浓度的3种多环芳烃对海胆细胞色素CYP17A1基因表达产生不同程度的影响。与对照组相比,暴露14 d后,苯并(a)芘20μg·L-1处理组对海胆CYP17A1基因的表达表现出抑制作用(P<0.05);暴露14 d后,3-甲基菲100μg·L-1处理组对海胆CYP17A1基因的表达表现出诱导作用(P<0.05);暴露14 d后,2-甲基蒽50μg·L-1处理组对海胆CYP17A1基因的表达表现出抑制作用(P<0.05)。2-甲基蒽对海胆CYP17A1基因表达的影响比苯并(a)芘、3-甲基菲的影响更为明显。In order to study the toxic effects of different polycyclic aromatic hydrocarbons(PAHs)on Strongylocentrotus intermedius,the effects of different concentrations of benzopyrene(1,5,20μg·L-1),3-methylphenanthrene(5,10,100μg·L-1)and 2-methylanthracene(5,10,50μg·L-1)on the expression of cytochrome CYP17A1 gene were investigated.The results showed that with the increase of exposure time,the three PAHs at different concentrations had different effects on the expression of CYP17A1 gene in Strongylocentrotus intermedius.Compared with control group,after exposure for 14 d,the expression of CYP17A1 gene was inhibited by benzopyrene in 20μg·L-1 group(P<0.05);after exposure for 14 d,the expression of CYP17A1 gene was induced by 3-methylphenanthrene in 100μg·L-1 group(P<0.05);after exposure for 14 d,the expression of CYP17A1 gene was inhibited by 2-methylanthracene in 50μg·L-1 group(P<0.05).The effects of 2-methylanthracene on cytochrome CYP17A1 gene expression of Strongylocentrotus intermedius are more obvious than benzopyrene and 3-methylphenanthrene.

关 键 词：苯并芘 3-甲基菲 2-甲基蒽 中间球海胆 细胞色素CYP17A1 基因表达 

分 类 号：X171.5[环境科学与工程—环境科学]