掺钕钇铝石榴石激光对人滋养层细胞表面抗原2的调控及口腔鳞癌细胞HN6增殖、侵袭和转移的影响  

Effects of Nd:YAG laser⁃regulated TROP2 on proliferation,invasion and metastasis of oral squamous cell carcinoma HN6 cell

作　　者：马浩然 龚爱秀[1] 汤根兄[1,2] MA Haoran;GONG Aixiu;TANG Genxiong(Department of Stomatology,Affiliated Children′s Hospital of Nanjing Medical University,Nanjing 210008,China)

机构地区：[1]南京医科大学附属儿童医院,南京210008 [2]国家卫健委抗体技术重点实验室,南京211166

出　　处：《实用医学杂志》2021年第2期175-181,共7页The Journal of Practical Medicine

基　　金：江苏省妇幼健康科研项目(编号:F201607);南京医科大学科技发展基金项目(编号:NMUB2019211)。

摘　　要：目的探讨激光对人滋养层细胞表面抗原2(TROP2)调控及对口腔鳞癌细胞HN6增殖、转移和侵袭的影响。方法体外培养口腔鳞癌细胞株HN6,用1064 nm Nd:YAG激光连续照射,检测24、48 h后激光对HN6细胞的增殖、划痕愈合、转移、侵袭的影响。检测Nd:YAG激光对HN6细胞中TROP2表达的影响。TROP2干扰质粒转染至HN6细胞后,检测Nd:YAG激光对HN6细胞增殖和侵袭的影响。结果低剂量和中剂量激光照射时,HN6细胞增殖能力增强,促划痕愈合程度和侵袭能力提高,TROP2表达较高(P<0.05);高剂量激光照射时,HN6细胞增殖受到抑制,划痕愈合明显减慢且侵袭能力受到抑制,TROP2表达较低(P<0.05);干扰TROP2表达后,Nd:YAG激光对HN6细胞增殖与侵袭无影响。结论较低剂量Nd:YAG激光照射促进HN6细胞的增殖、转移和侵袭,高剂量Nd:YAG激光照射抑制HN6细胞的增殖、转移和侵袭。Nd:YAG激光通过调控TROP2的表达影响HN6的增殖及侵袭转移。Objective To investigate the effects of Nd:YAG laser on TROP2 and the HN6 proliferation,migration and invasion of oral squamous cell carcinoma cells.Methods HN6 cell lines were cultured in vitro,and the effects of laser irradiation on proliferation ability,scratch healing and migration and invasion of HN6 cells were detected,with continuous irradiation by pulsed 1064 nm Nd:YAG laser after 24 and 48 h.TROP2 expres⁃sion was detected by the effects of Nd:YAG laser on HN6 cells.Then,TROP2 knockdown plasmid was constructed and transfected into HN6 cells.The effects of Nd:YAG laser were investigated on the proliferation and invasion of HN6 cells.Results With low and medium dose laser irradiation,the proliferation of HN6 cells was stronger,the degree of promoting scratch healing and the invasion ability were improved,and TROP2 expression was higher(P<0.05).But under high dose laser irradiation,HN6 cell proliferation was inhibited,the degree of scratch healing was slowed down,the invasion ability was inhibited,and TROP2 expression was lower(P<0.05).After After interfering with TROP2 expression,the effects of Nd:YAG laser on the proliferation and invasion of HN6 cells were disappeared.Conclusions The lower dose of Nd:YAG laser irradiation promotes the proliferation,migration and invasion of HN6 cells,while the higher dose of Nd:YAG laser irradiation inhibits the proliferation,migration and invasion of HN6 cells.By regulating the expression of TROP2,the Nd:YAG laser affects the prolifera⁃tion,invasion and metastasis of HN6 cells.

关 键 词：ND:YAG激光 TROP2 口腔鳞状细胞 增殖 转移 侵袭 

分 类 号：R782[医药卫生—口腔医学]