微小RNA-106a靶向PTEN通路对胃癌细胞的增殖与凋亡的调控作用  

Regulation of microRNA-106a Targeting PTEN Pathway on The Proliferation andApoptosis of Gastric Cancer Cells

作　　者：李丽 张静 郭锦涛 LI Li;ZHANG Jing;GUO Jintao(Xinyang Central Hospital,Xinyang,464000)

机构地区：[1]信阳市中心医院,464000

出　　处：《实用癌症杂志》2021年第1期20-23,27,共5页The Practical Journal of Cancer

基　　金：河南省高等学校重点科研计划项目(编号:18A320072)。

摘　　要：目的探讨微小RNA-106a(miR-106a)靶向磷酸酶和张力蛋白同源物(PTEN)通路对胃癌细胞的增殖与凋亡的调控作用。方法对数生长期的胃癌BGC-823细胞为三组:miR-106a组、对照组与空白组,分别转染miR-106a mimic、miR-NC与等体系的磷酸盐缓冲液。采用CCK法检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell小室实验检测细胞迁移与侵袭,Western blot检测PTEN蛋白表达,qRT-PCR检测miR-106a表达。结果细胞转染后24 h与48 h,miR-106a组的miR-106a相对表达量显著高于空白组和对照组(P<0.05);与空白组和对照组相比,miR-106a组的细胞增殖指数、细胞迁移指数、侵袭指数PTEN蛋白相对表达水平显著降低(P<0.05),细胞凋亡指数显著增加(P<0.05),而前两组相比差异无统计学意义(P>0.05);与24 h时相比,48 h时三组细胞凋亡、侵袭、迁移指数以及PTEN蛋白相对表达水平显著降低(P<0.05),其余指标仅miR-106a组两个时间点相比差异具有统计学意义(P<0.05),而空白组和对照组相比均不具有统计学意义(P>0.05)。结论miR-106a过表达可通过靶向调控PTEN信号通路,抑制胃癌细胞增殖、迁移与侵袭,并促进细胞凋亡。Objective To investigate the regulatory effects of microRNA-106a(miR-106a)targeting phosphatase and tensin homologue-deleted chromosome ten gene(PTEN)pathway on the proliferation and apoptosis of gastric cancer cells.Methods The gastric cancer BGC-823 cells were divided into miR-106a group,the control group and the blank group,miR-106a group and the control group were transfected with miR-106a mimic,miR-NC,and the blank group were transfected with the same volume of phosphate buffer solutions.Cell proliferation were detected by CCK method,apoptosis were detected by flow cytometry,cell migration and invasion were detected by Transwell chamber experiment,PTEN protein protein expression were detected by Western blot,and miR-106a expression were detected by qRT-PCR.Results The relative expression levels of miR-106a in miR-106a group were significantly higher than those in the blank group and the control group at 24 and 48 hours after cell transfection(P<0.05).The proliferation index,cell migration index,invasion index the relative expression levels of PTEN protein were significantly reduced(P<0.05),and the apoptosis index were significantly increased(P<0.05).There was no significant difference between the first 2 groups(P>0.05);Compared with 24 h,the apoptosis,invasion,migration index,and relative expression levels of PTEN protein in the three groups were significantly reduced at 48 h(P<0.05).The remaining indicators were statistically different only at the two time points of the miR-106a group(P<0.05),but the blank group was not statistically significant compared with the control group(P>0.05).Conclusion Overexpression of miR-106a can target the inhibition of PTEN signaling pathway activation,thereby inhibitthe proliferation,metastasis and invasion of gastric cancer cells,and promote apoptosis.

关 键 词：PTEN 胃癌 微小RNA-106a 细胞增殖 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]