广泛型侵袭性牙周炎患者牙根形态异常与相关致病基因的关联  

Association between root abnormalities and related pathogenic genes in patients with generalized aggressive periodontitis

作　　者：刘建[1] 王宪娥[1] 吕达[1] 乔敏[1] 张立[1] 孟焕新[1] 徐莉[1] 毛铭馨 LIU Jian;WANG Xian-e;LV Da;QIAO Min;ZHANG Li;MENG Huan-xin;XU Li;MAO Ming-xin(Department of Periodontology,Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China)

机构地区：[1]北京大学口腔医学院·口腔医院,牙周科国家口腔疾病临床医学研究中心口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室,北京100081

出　　处：《北京大学学报（医学版）》2021年第1期16-23,共8页Journal of Peking University:Health Sciences

摘　　要：目的:探索广泛型侵袭性牙周炎(generalized aggressive periodontitis,GAgP)患者牙根形态异常与骨代谢或牙根发育相关基因多态性的关联。方法:纳入179例GAgP患者,平均(27.23±5.19)岁,男∶女=67∶112,平均存留牙数(26.80±1.84)颗。采用基于基质辅助激光解吸电离飞行时间质谱技术进行9个与骨代谢和牙根发育相关基因的13个单核苷酸多态性位点(single nucleotide polymorphisms,SNPs)的基因型检测。采用全口根尖片评判牙根形态异常,包括锥根、细长根、冠根比例失调、弯曲根、融合根、后牙根形态异常,分析13个SNPs位点不同基因型根形态异常牙的数量及发生率。结果:GAgP患者根形态异常牙构成比为14.49%(695/4798颗),平均(3.88±3.84)颗。维生素D受体(vitamin D receptor,VDR)基因rs2228570位点的CC、CT、TT基因型患者根形态异常牙数量分别为(4.66±4.10)、(3.71±3.93)和(2.68±2.68)颗,CC基因型和TT基因型之间差异有统计学意义(t=2.62,P=0.01)。降钙素受体(calcitotin receptor,CTR)基因rs2283002位点CC、CT、TT基因型患者根形态异常数分别为(5.02±3.70)、(3.43±3.95)、(3.05±3.12)颗,CC基因型的根形态异常发病率高于CT和TT基因型(87.86%vs.65.26%和63.64%,P=0.006,adjusted OR=3.71,95%CI:1.45~9.50)。结论:VDR rs2228570及CTR rs2283002位点可能与广泛型侵袭性牙周炎患者牙根形态异常的发生有关,值得进一步研究。Objective:To explore the association between the abnormal root morphology and bone metabolism or root development related gene polymorphism in patients with generalized aggressive periodontitis.Methods:In the study,179 patients with generalized aggressive periodontitis were enrolled,with an average age of(27.23±5.19)years,male/female=67/112.The average number of teeth remaining in the mouth was(26.80±1.84).Thirteen single nucleotide polymorphisms(SNPs)of nine genes which related to bone metabolism and root development were detected by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS).Root abnormalities were identified using periapical radiographs.The abnormal root morphology included cone-rooted teeth,slender-root teeth,short-rooted teeth,curved-rooted teeth,syncretic-rooted molars,and molar root abnormalities.The number of teeth and incidence of abnormal root morphology in different genotypes of 13 SNPs were analyzed.Results:The constituent ratio of root with root abnormality in GAgP patients was 14.49%(695/4798).The average number of teeth with abnormal root morphology in GAgP was(3.88±3.84).The average number of teeth with abnormal root morphology in CC,CT and TT genotypes in vitamin D receptor(VDR)rs2228570 was(4.66±4.10),(3.71±3.93)and(2.68±2.68).There was significant difference between TT genotype and CC genotype(t=2.62,P=0.01).The average number of root morphological abnormalities in CC,CT and TT genotypes of Calcitotin Receptor(CTR)gene rs2283002 was(5.02±3.70),(3.43±3.95),and(3.05±3.12).The incidence of root morphological abnormalities in CC genotype was higher than that in the patients with CT and TT,and the difference was statistically significant(87.86%vs.65.26%&63.64%,P=0.006,adjusted OR=3.71,95%CI:1.45-9.50).There was no significant difference in the incidence of abnormal root morphology between CT and TT genotypes.Conclusion:VDR rs2228570 and CTR rs2283002 may be associated with the occurrence of abnormal root morphology in patients with generalized

关 键 词：侵袭性牙周炎 牙根形态异常 基因 单核苷酸多态性 

分 类 号：R394.3[医药卫生—医学遗传学]