中宁-格尔木产区宁杞一号干果蛋白质组学研究  

Proteomics Study of Ningqi No.1 Dried Fruit in Zhongning-Golmud Producing Area

作　　者：孙西予 牛思思 赵廷彬 赵丹青[5] 余君伟 乔长晟[1,2,4,7] SUN Xi-yu;NIU Si-si;ZHAO Ting-bin;ZHAO Dan-qing;YU Jun-wei;QIAO Chang-sheng(School of Bioengineering,Tianjin University of Science and Technology,Tianjin 300457,China;Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China;Qiyuantang(Tianjin)Biotrans Bioengineering Co.,Ltd.,Tianjin 300453,China;Tianjin Huizhi Baichuan Biotechnology Co.,Ltd.,Tianjin 300457,China;Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,Ningxia,China;Ningxia Zhongnong Aisen Detection Co.,Ltd.,Zhongning 755100,Ningxia,China;Tianjin Engineering Technology Center for Microbial Metabolism and Fermentation Process Control,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室,天津300457 [3]杞源堂(天津)生物工程有限公司,天津300453 [4]天津慧智百川生物技术有限公司,天津300457 [5]宁夏农林科学院,宁夏银川750002 [6]宁夏中农艾森检测有限公司,宁夏中宁755100 [7]天津市微生物代谢与发酵过程控制工程技术中心,天津300457

出　　处：《食品研究与开发》2021年第3期163-169,共7页Food Research and Development

基　　金：天津市科技计划项目(17YFCZZC00310);天津市科技计划项目(17PTSYJC00080);宁夏农林科学院“对外科技合作”项目(DWX-2018015);宁夏回族自治区重点研发计划重大(重点)项目(2019BFH02020);宁夏回族自治区重点研发计划一般项目(2018BBF53006)。

摘　　要：通过非标记定量(label-free)蛋白质组学技术对宁夏中宁和青海格尔木两个产地的枸杞干果样品进行蛋白组学比较。结果表明两种样品共鉴别出蛋白质2477个,中宁产区样品中含蛋白质1942个(其中174个为中宁样品特有),格尔木产区样品中含蛋白质2065个(其中297个为格尔木样品特有);另外中宁产地的样品和格尔木产地的样品相比有86个蛋白质的表达量下调,69个蛋白质的表达量上调。对样品蛋白质进行层次聚类分析,证明两产地样品在蛋白质组学上有明显差异。利用GO数据库和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)数据库对所有差异蛋白质进行检索,筛选后得到28个目标差异蛋白质,其中包括:4个金属离子转运蛋白、6个热休克蛋白、3个几丁质酶、4个磷酸肌醇转化酶、2个过氧化氢酶、3个与植物激素调节有关的蛋白质、5个与卟啉和叶绿素代谢相关的酶、1个核糖核苷二磷酸激酶。Label-free technology was used to make a comparison of proteomics between the Ningqi No.1 Lycium barbarum L.dried fruit samples from Zhongning,Ningxia and Golmud,Qinghai.The results showed that a total of 2477 proteins were identified from the two samples,including 1942 proteins from Zhongning samples(174 of which were endemic to Zhongning samples),and 2065 proteins from Golmud samples(297 of which were endemic to Golmud samples).In addition,compared with that of Golmud sample,86 proteins in Zhongning sample were down-regulated and 69 proteins were up-regulated.The hierarchical clustering analysis of the samples showed that there were significant differences in proteomics between the samples from the two producing areas.All the differentially expressed proteins were detected by Gene Ontology(GO) database and KEGG database, and totally 28 target differentially expressed proteins were focused,which include 4 metal ion transporters, 6 heat shock proteins, 3 chitinases, 4 phosphoinositol converting enzymes, 2 catalases, 3 proteins involved in plant hormone regulation, 5 enzymes involved in porphyrin and chlorophyll metabolism,1 nucleoside diphosphate kinase.

关 键 词：蛋白质组学 非标记定量蛋白质组学(label-free) GO数据库 京都基因与基因组百科全书(KEGG) 宁杞一号 

分 类 号：TS201.21[轻工技术与工程—食品科学]