LAP^(+)CD4^(+)T细胞在胃癌外周血中的分布情况及临床意义  

The distribution of LAP^(+)CD4^(+)T cells in peripheral blood of gastric cancer and its clinical significance

作　　者：钟武[1] 刘传渊[1] 张磊[1] 刘红权[1] 易建中[1] 方传发[1] 张磊昌[2] ZHONG Wu;LIU Chuanyuan;ZHANG Lei;LIU Hongquan;YI Jianzhong;FANG Chuanfa;ZHANG Leichang(Department of Gastrointestinal Hernia Surgery,Ganzhou People's Hospital in Jiangxi Province,Ganzhou 341000,China;Department of Proctology,Jiangxi Hospital of Traditional Chinese Medicine,Nanchang 330000,China)

机构地区：[1]江西省赣州市人民医院胃肠疝外科,江西赣州341000 [2]江西省中医院肛肠科,江西南昌330000

出　　处：《中国现代医生》2020年第35期16-19,共4页China Modern Doctor

基　　金：江西省卫生计生委科技计划(20174046);江西省自然科学基金(20181BAB205059)。

摘　　要：目的检测胃癌患者外周血中LAP^(+)CD4^(+)T细胞和CD4^(+)CD25^(+)Treg细胞的分布比例,初步研究LAP^(+)CD4^(+)T细胞与病理因素的相关性及在胃癌发生、进展中的作用。方法收集2018年1月~2019年6月我院胃肠疝外科收治的50例胃癌患者术前及术后临床病理资料,收集术前患者的外周血标本,收集同期50例健康志愿者外周血作为对照组。流式细胞技术检测LAP^(+)CD4^(+)T细胞和CD4^(+)CD25^(+)Treg的分布比例,比较外周血中胃癌患者和健康志愿者LAP^(+)CD4^(+)T细胞的分布比例差异;Pearson法分析LAP^(+)CD4^(+)T细胞与临床病理因素及CD4^(+)CD25^(+)Treg细胞的相关性。结果外周血LAP^(+)CD4^(+)T细胞占CD4^(+)T细胞的比例,胃癌患者为[7.58(6.23,8.22)%]高于健康志愿者的[1.76(0.79,2.35)%](P<0.001);Pearson相关性分析结果显示,外周血中LAP^(+)CD4^(+)T细胞与CD4^(+)CD25^(+)Treg细胞分布比例呈正相关(r=0.629,P<0.001);肿瘤TNM分期越晚,LAP^(+)CD4^(+)T细胞占CD4^(+)T细胞比例越高(P<0.001);有远处转移患者LAP^(+)CD4^(+)T细胞占CD4^(+)T细胞比例高于无转移者(P<0.001)。结论LAP^(+)CD4^(+)T细胞在胃癌患者外周血中比例升高,可能参与了胃癌的发生、进展,起到促进肿瘤生长、转移的作用。Objective To detect the distribution ratio of LAP^(+)CD4^(+)T cells and CD4^(+)CD25^(+)Treg cells in peripheral blood of patients with gastric cancer and preliminarily investigate the correlation between LAP^(+)CD4^(+)T cells and clinicopathological factors as well as their roles in the occurrence and development of gastric cancer.Methods Preoperative and postoperative clinicopathological data of 50 patients with gastric cancer admitted to the Department of Gastrointestinal Surgery in our hospital from January 2018 to June 2019 were collected.Peripheral blood samples of preoperative patients were collected,and peripheral blood samples of 50 healthy volunteers with matching clinical features were collected as the control group.Flow cytometry was used to detect the distribution ratio of LAP^(+)CD4^(+)T cells and CD4^(+)CD25^(+)Treg.The difference in the distribution ratio of LAP^(+)CD4^(+)T cells in peripheral blood between gastric cancer patients and healthy volunteers was compared.Pearson assay was used to analyze the correlation between LAP^(+)CD4^(+)T cells and clinicopathological factors as well as CD4^(+)CD25^(+)Treg cells.Results The proportion of peripheral blood LAP^(+)CD4^(+)T cells in CD4^(+)T cells of patients with gastric cancer accounted for(7.58[6.23,8.22]%),which was higher than that of healthy volunteers(1.76[0.79,2.35]%)(P<0.001).Pearson correlation analysis showed that the LAP^(+)CD4^(+)T cells in the peripheral blood were positively related to CD4^(+)CD25^(+)Treg cells(r=0.629,P<0.001).The later TNM staging,the higher the proportion of LAP^(+)CD4^(+)T cells in CD4^(+)T cells(P<0.001),The percentage of CD4^(+)T cells in patients with distant metastasis was higher than that in patients without metastasis(P<0.001).Conclusion The increased proportion of LAP^(+)CD4^(+)T cells in peripheral blood of patients with gastric cancer may be involved in the occurrence and development of gastric cancer and play a role in promoting tumor growth and metastasis.

关 键 词：LAP^(+)CD4^(+)T细胞 调节性T细胞 胃癌 肿瘤微环境 

分 类 号：R735.2[医药卫生—肿瘤]