光学分子探针FITC-CSPLNTRFC对乳腺癌细胞Bcap-37的特异性和靶向性  

Specificity and Targeting of Optical Molecular Probe FITC-CSPLNTRFC on BreastCancer Cells Bcap-37

作　　者：郭超[1] 左琪 杨晓峰[2] 张帆[1] GUO Chao;ZUO Qi;YANG Xiaofeng;ZHANG Fan(Department of Microbiology and Immunology,Shanxi Medical University,Taiyuan 030001,China;Department of Urology,The First Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学微生物学与免疫学教研室,太原030001 [2]山西医科大学第一医院泌尿外科,太原030001

出　　处：《肿瘤防治研究》2021年第1期24-30,共7页Cancer Research on Prevention and Treatment

基　　金：山西省晋中市科技攻关项目(S1610);山西综改示范区晋中开发区科技重点研发计划。

摘　　要：目的研究光学分子探针FITC-BCSP1对乳腺癌细胞Bcap-37的特异性和靶向性。方法固相合成法制备探针FITC-BCSP1和阴性对照探针FITC-svBCSP1。MTT法检测两种探针对乳腺癌Bcap-37细胞的毒性。流式细胞术和荧光倒置显微镜鉴定FITC-BCSP1与Bcap-37细胞结合的特异性。光学分子成像仪验证FITC-BCSP1对Bcap-37细胞荷瘤裸鼠模型移植瘤的特异性和靶向性。结果质谱和高效液相色谱法鉴定,所合成探针纯度≥98%。探针FITC-BCSP1和对照探针FITC-svBCSP1在50~300μmol/L浓度内对Bcap-37细胞增殖、活性无影响(IR%≤30%)。流式细胞术显示Bcap-37细胞中FITC-BCSP1标记的细胞百分比明显高于其他细胞(均P<0.001);FITC-BCSP1标记的Bcap-37细胞百分比明显高于对照组(P<0.001)。荧光倒置显微镜下观察到,FITC-BCSP1标记Bcap-37细胞中有大量荧光细胞,阳性率为100%,而FITC-svBCSP1组阳性率仅1%。Bcap-37细胞荷瘤裸鼠模型体内验证实验显示,FITC-BCSP1能特异性富集在移植瘤组织。结论光学分子探针FITC-BCSP1有良好的乳腺癌细胞特异性及靶向性,可用于乳腺癌早期诊断研究。Objective To investigate the specificity and targeting abilities of FITC-CSPLNTRFC peptide FITC-BCSP1 optical molecular probe on breast cancer cells Bcap-37.Methods Probe FITC-BCSP1 and negative control probe FITC-svBCSP1 were prepared by solid phase synthesis.MTT assay was used to determine the toxicity of the two probes on breast cancer cells Bcap-37.The specificity of the binding of FITC-BCSP1 probe to Bcap-37 cells was identified by flow cytometry and fluorescent inversed microscopy.The specificity and targeting abilities of FITC-BCSP1 probe for transplantation tumor in Bcap-37 cells tumorbearing nude mice model were tested by optical molecular imager.Results The purity of the synthesized probe was more than 98%,identified by mass spectrometry and high performance liquid chromatography.FITC-BCSP1 and FITC-svBCSP1 probes had no effect on proliferation and activity of Bcap-37 cells at the concentrations of 50-300 mol/L(IR%≤30%).FCM results showed that the percentage of FITC-BCSP1-labeled cells in Bcap-37 cells was significantly higher than that in other cells(all P<0.001),and the percentage of FITC-BCSP1-labeled Bcap-37 cells was significantly higher than that of the control group(P<0.001).It was observed under inverted fluorescence microscope that there were a large number of fluorescent cells in FITC-BCSP1-labeled Bcap-37 cells,with a positive rate of 100%,while the positive rate of FITC-svBCSP1 group was only 1%.In vivo assay with Bcap-37 cells tumor-bearing nude mice model showed that FITCBCSP1 probe could be specifically enriched in the transplantation tumor tissue.Conclusion The optical molecular probe FITC-BCSP1 has good specificity and targeting abilities on breast cancer cells and can be used in the early diagnosis of breast cancer.

关 键 词：乳腺癌 靶向肽 光学分子探针 特异性 靶向性 

分 类 号：R737.9[医药卫生—肿瘤]