环状RNA Hsa_circ_0026352在乳腺癌中的表达及临床意义  

Expression of Circular RNA Hsa_circ_0026352 in Breast Cancer and Its Clinical Significance

作　　者：张旭[1,2] 马芳 纳玮 李晓菡[1,2] 黄琦 于晶晶[1,2] 王嘉 王立斌[1,2] ZHANG Xu;MA Fang;NA Wei;LI Xiaohan;HUANG Qi;YU Jingjing;WANG Jia;WANG Libin(Department of Beijing National Biochip Research Center Sub-center in Ningxia,General Hospital of Ningxia Medical University,Yinchuan 750004,China;College of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China;Department of Microbiological Examination,Yinchuan City Center for Disease Control and Prevention,Yinchuan 750011,China)

机构地区：[1]宁夏医科大学总医院生物芯片北京国家工程研究中心宁夏分中心,银川750004 [2]宁夏医科大学临床医学院,银川750004 [3]银川市疾病预防控制中心微生物检验科,银川750011

出　　处：《肿瘤防治研究》2021年第1期43-48,共6页Cancer Research on Prevention and Treatment

基　　金：宁夏高等学校优秀青年科学研究项目(NGY2018-91);宁夏自然科学基金(2018AAC03156);国家自然科学基金(81860470);宁夏自治区重点研发计划(2019BFH02012);宁夏科技创新领军人才项目(KJT2019003);宁夏高等学校一流学科建设(宁夏医科大学国内一流学科临床医学)项目(NXYIXK2017A05)。

摘　　要：目的探讨Hsa_circ_0026352在乳腺癌组织及外周血中的表达及其与乳腺癌患者临床特征的相关性,评价其作为乳腺癌诊断标志物的价值。方法通过人circRNA芯片筛选在乳腺癌组织中差异表达的circRNA,qRT-PCR技术验证Hsa_circ_0026352在乳腺癌组织与外周血中的相对表达,circPrimer 1.2软件绘制circRNA结构图,采用单因素t检验、ANOVA分析、曲线回归分析和ROC曲线分析Hsa_circ_0026352用于外周血诊断乳腺癌的价值。结果Hsa_circ_0026352在乳腺癌组织及外周血中显著低表达(P<0.01),在组织及外周血样本中检测乳腺癌的AUC分别为0.881和0.826(P<0.01)。Hsa_circ_0026352在乳腺癌患者外周血中的相对表达与CA153水平显著负相关(P<0.05)。其用于外周血检测ER阳性、乳腺癌分期、乳腺肿瘤转移的AUC分别为0.710(P<0.01)、0.771(P<0.01)和0.722(P<0.01)。结论Hsa_circ_0026352具备成为乳腺癌诊断分子标志物的潜力。Objective To investigate the correlation between the expression of Hsa_circ_0026352 and the clinical characteristics of breast cancer(BC)patients,to evaluate the value of Hsa_circ_0026352 as a diagnostic marker of breast cancer.Methods Human circRNA microarray was used to screen the different expression of circRNAs in BC tissues.qRT-PCR was used to verify the expression of Hsa_circ_0026352 in BC tissue and peripheral blood.CircRNA structure were performed by circPrimer1.2 software.T-test,ANOVA analysis,curve regression analysis and ROC curve analysis were performed to determine the diagnostic values of Hsa_circ_0026352.Results Hsa_circ_0026352 was significantly down-regulated in both breast cancer tissues and peripheral blood(P<0.01).The AUC of Hsa_circ_0026352 were 0.881 in tissues and 0.826 in peripheral blood(both P<0.01).The relative expression of Hsa_circ_0026352 in peripheral blood of BC cancer patients was negatively correlated with CA153 level(P<0.05).The AUC of Hsa_circ_0026352 in peripheral blood of patients with ER positive,early stage breast cancer and breast neoplasm metastasis were 0.710,0.771 and 0.722(all P<0.01),respectively.Conclusion Hsa_circ_0026352 could be a novel predictive biomarker for diagnosis of BC.

关 键 词：环状RNA 乳腺癌 标志物 环状RNA芯片 

分 类 号：R737.9[医药卫生—肿瘤]