雌激素增强失血性休克大鼠肠系膜淋巴管的收缩功能  

Estrogen treatment enhances mesenteric lymphatic contractility in rats after hemorrhagic shock

作　　者：张立民[1] 冯妞妞 赵晨[1] 张玉平[1] 牛春雨 赵自刚[1] ZHANG Li-min;FENG Niu-niu;ZHAO Chen;ZHANG Yu-ping;NIU Chun-yu;ZHAO Zi-gang(Institute of Microcirculation and Basic Medical College of Hebei North University,Zhangjiakou 075000,China;Basic Medical College of Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北北方学院微循环研究所/基础医学院,河北张家口075000 [2]河北医科大学基础医学院,河北石家庄050017

出　　处：《中国病理生理杂志》2021年第1期66-71,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81670446)。

摘　　要：目的:观察17β-雌二醇(E2)对失血性休克大鼠肠系膜淋巴微循环和离体肠系膜淋巴管收缩性的作用及其与淋巴管平滑肌细胞(LSMCs)内外钙离子浓度([Ca2+])差的关系。方法:雄性Wistar大鼠随机均分为假手术组、休克组和休克+E2组,建立失血性休克模型[(40±2)mmHg维持1.5 h,液体复苏],休克+E2组在液体复苏时皮下注射E2(2 mg/kg);液体复苏后3 h或相应时点,观察回肠下段活体肠系膜淋巴微循环。制备离体肠系膜淋巴管环,观察舒张末期口径、收缩末期口径、收缩频率(CF)及最大被动舒张管径,评价淋巴管收缩性,同时记录淋巴管收缩过程中LSMCs内外[Ca2+]差的变化。结果:雌激素治疗显著提高失血性休克大鼠活体肠系膜淋巴管的CF、总收缩分数和动力学指数,离体肠系膜淋巴管的CF、泵流分数及LSMCs内外[Ca2+]差(P<0.05)。结论:雌激素增强了失血性休克大鼠肠系膜淋巴管的收缩功能,该作用与提高LSMCs内外[Ca2+]差有关。AIM:To investigate the effects of 17β-estradiol(E2)treatment on the mesenteric lymphatic microcirculation and isolated lymphatic contractility in rats after hemorrhagic shock,and to explore the relationship between contractility and the difference between intra-and extracellular calcium ion concentrations([Ca2+])of lymphatic smooth muscle cells(LSMCs).METHODS:Male Wistar rats were divided into sham group,shock group and shock+E2 group.The rats were subjected to hemorrhage[(40±2)mmHg for 90 min]and resuscitation with or without subcutaneous injection of E2(2 mg/kg).After resuscitation for 3 h,the mesenteric lymphatic microcirculation in vivo was observed.Moreover,the isolated mesenteric microlymphatic rings were prepared for the observations of lymphatic contractility evaluated by the indexes including end-systolic diameter,end-diastolic diameter,contraction frequency(CF)and passive diameter.Meanwhile,the difference between intra-and extracellular[Ca2+]of LSMCs was recorded during lymphatic contraction.RESULTS:Treatment with E2 significantly enhanced the CF,total contractile fraction and lymphatic dynamics index in vivo in the rats after hemorrhagic shock,and increased the CF,the fractional pump flow and the difference between intraand extracellular[Ca2+]of LSMCs in isolated lymphatics from the shocked rats(P<0.05).CONCLUSION:Estrogen treatment enhances lymphatic contractility in rats after hemorrhagic shock,which is related to enhancement of difference between intra-and extracellular[Ca2+]of LSMCs.

关 键 词：雌激素 失血性休克 肠系膜淋巴管 钙离子 淋巴管平滑肌细胞 

分 类 号：R364.1[医药卫生—病理学] R363.2[医药卫生—基础医学]