Modules for in vitro metabolic engineering:Pathway assembly for biobased production of value-added chemicals  

作　　者：Hironori Taniguchi Kenji Okano Kohsuke Honda 

机构地区：[1]Synthetic Bioengineering Lab,Department of Biotechnology,Graduate School of Engineering,Osaka University,2-1 Yamadaoka,Suita,Osaka,565-0871,Japan

出　　处：《Synthetic and Systems Biotechnology》2017年第2期65-74,共10页合成和系统生物技术（英文）

基　　金：Research in our laboratory was supported in part by the Japan Science and Technology Agency(PRESTO,CREST,and A-STEP programs)and the Japan Society for the Promotion of Science(KAKENHI program).

摘　　要：Bio-based chemical production has drawn attention regarding the realization of a sustainable society.In vitro metabolic engineering is one of the methods used for the bio-based production of value-added chemicals.This method involves the reconstitution of natural or artificial metabolic pathways by assembling purified/semi-purified enzymes in vitro.Enzymes from distinct sources can be combined to construct desired reaction cascades with fewer biological constraints in one vessel,enabling easier pathway design with high modularity.Multiple modules have been designed,built,tested,and improved by different groups for different purpose.In this review,we focus on these in vitro metabolic engineering modules,especially focusing on the carbon metabolism,and present an overview of input modules,output modules,and other modules related to cofactor management.

关 键 词：In vitro metabolic engineering Module GLYCOLYSIS Pentose phosphate pathway Cofactor management 

分 类 号：O15[理学—数学]