Engineered polyketides:Synergy between protein and host level engineering  

作　　者：Jesus F.Barajas Jacquelyn M.Blake-Hedges Constance B.Bailey Samuel Curran Jay.D.Keasling 

机构地区：[1]Department of Energy Agile BioFoundry,Emeryville,CA,USA [2]Department of Chemistry,University of California,Berkeley,Berkeley,CA 94720,USA [3]Joint BioEnergy Institute,Emeryville,CA 94608,USA [4]Physical Biosciences Division,Lawrence Berkeley National Laboratory,Berkeley,CA 94720,USA [5]Comparative Biochemistry Graduate Group,University of California,Berkeley,Berkeley,CA 94720,USA [6]QB3 Institute,University of California,Berkeley,Emeryville,CA 94608,USA [7]Department of Chemical&Biomolecular Engineering,Department of Bioengineering,University of California,Berkeley,Berkeley,CA 94720,USA [8]Novo Nordisk Foundation Center for Biosustainability,Technical University Denmark,DK2970 Horsholm,Denmark

出　　处：《Synthetic and Systems Biotechnology》2017年第3期147-166,共20页合成和系统生物技术（英文）

基　　金：This work was funded by the Joint BioEnergy Institute(JBEI),which is funded by the U.S.Department of Energy,Office of Science,Office of Biological and Environmental Research,under Contract DE-AC02-05CH11231;by the National Science Foundation under awards MCB-1442724,NSF-GRFP DGE-1106400 and CBET-1437775;as part of the Co-Optimization of Fuels&Engines(Co-Optima)project sponsored by the U.S.Department of Energy(DOE)Office of Energy Efficiency and Renewable Energy(EERE);Bioenergy Technologies and Vehicle Technologies Offices,and by the DOE Agile-Biofoundry(https://agilebiofoundry.org)supported by the U.S.Department of Energy,Energy Efficiency and Renewable Energy,Bioenergy Technologies Office,through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U.S.Department of Energy.The United States Government retains and the publisher,by accepting the article for publication,acknowledges that the United States Government retains a nonexclusive,paid-up,irrevocable,world-wide license to publish or reproduce the published form of this manuscript,or allowothers to do so,for United States Government purposes.Additional funding was provided by the National Science Foundation Graduate Research Fellowship under Grant No.(DGE 1106400).

摘　　要：Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities.Polyketides encompass one of the largest classes of chemically diverse natural products.With thousands of known polyketides,modular polyketide synthases(PKSs)share a particularly attractive biosynthetic logic for generating chemical diversity.The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds.In this review,we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures,and we examine future applications of PKSs in the production of medicines,fuels and other industrially relevant chemicals.

关 键 词：POLYKETIDE Polyketide synthase Natural products Commodity chemical Metabolic engineering Synthetic biology 

分 类 号：F42[经济管理—产业经济]