Protein engineering of oxidoreductases utilizing nicotinamide-based coenzymes,with applications in synthetic biology  

作　　者：Chun You Rui Huang Xinlei Wei Zhiguang Zhu Yi-Heng Percival Zhang 

机构地区：[1]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,32 West 7th Avenue,Tianjin Airport Economic Area,Tianjin,300308,People's Republic of China [2]Biological Systems Engineering Department,Virginia Tech,304 Seitz Hall,Blacksburg,VA 24061,USA

出　　处：《Synthetic and Systems Biotechnology》2017年第3期208-218,共11页合成和系统生物技术（英文）

基　　金：This study was mainly supported by the Key Research Program of the Chinese Academy of Sciences(Grant No.ZDRW-ZS-2016-3);1000-youth program of China to CY and the National Natural Science Foundation of China(Grant No.31600636);Funds were partially provided by the DOE EERE award(DE-EE0006968)to YPZ.

摘　　要：Two natural nicotinamide-based coenzymes(NAD and NADP)are indispensably required by the vast majority of oxidoreductases for catabolism and anabolism,respectively.Most NAD(P)-dependent oxidoreductases prefer one coenzyme as an electron acceptor or donor to the other depending on their different metabolic roles.This coenzyme preference associated with coenzyme imbalance presents some challenges for the construction of high-efficiency in vivo and in vitro synthetic biology pathways.Changing the coenzyme preference of NAD(P)-dependent oxidoreductases is an important area of protein engineering,which is closely related to product-oriented synthetic biology projects.This review focuses on the methodology of nicotinamide-based coenzyme engineering,with its application in improving product yields and decreasing production costs.Biomimetic nicotinamide-containing coenzymes have been proposed to replace natural coenzymes because they are more stable and less costly than natural coenzymes.Recent advances in the switching of coenzyme preference from natural to biomimetic coenzymes are also covered in this review.Engineering coenzyme preferences from natural to biomimetic coenzymes has become an important direction for coenzyme engineering,especially for in vitro synthetic pathways and in vivo bioorthogonal redox pathways.

关 键 词：Coenzyme engineering Nicotinamide-based coenzymes NAD NADP Protein engineering Synthetic biology Biomimetic coenzymes 

分 类 号：O63[理学—高分子化学]