Smart biomaterials: Surfaces functionalized with proteolytically stable osteoblast-adhesive peptides  

作　　者：Annj Zamuner Paola Brun Michele Scorzeto Giuseppe Sica Ignazio Castagliuolo Monica Dettin 

机构地区：[1]Department of Industrial Engineering,University of Padova,Via F.Marzolo 9,35131,Padova,Italy [2]Department of Molecular Medicine,University of Padova,Via A.Gabelli 63,35121,Padova,Italy [3]Department of Biomedical Sciences,University of Padova,Via U.Bassi 58/B,35131,Padova,Italy

出　　处：《Bioactive Materials》2017年第3期121-130,共10页生物活性材料（英文）

摘　　要：Engineered scaffolds for bone tissue regeneration are designed to promote cell adhesion,growth,proliferation and differentiation.Recently,covalent and selective functionalization of glass and titanium surfaces with an adhesive peptide(HVP)mapped on[351e359]sequence of human Vitronectin allowed to selectively increase osteoblast attachment and adhesion strength in in vitro assays,and to promote osseointegration in in vivo studies.For the first time to our knowledge,in this study we investigated the resistance of adhesion sequences to proteolytic digestion:HVP was completely cleaved after 5 h.In order to overcome the enzymatic degradation of the native peptide under physiological conditions we synthetized three analogues of HVP sequence.A retro-inverted peptide D-2HVP,composed of D amino acids,was completely stable in serum-containing medium.In addition,glass surfaces functionalized with D-2HVP increased human osteoblast adhesion as compared to the native peptide and maintained deposition of calcium.Interestingly,D-2HVP increased expression of IBSP,VTN and SPP1 genes as compared to HVP functionalized surfaces.Total internal reflection fluorescence microscope analysis showed cells with numerous filopodia spread on D-2HVP-functionalized surfaces.Therefore,the D-2HVP sequence is proposed as new osteoblast adhesive peptide with increased bioactivity and high proteolytic resistance.

关 键 词：Adhesive sequences Retro-inverso peptides Surface grafting Proteolytic degradation OSTEOBLAST TIRF 

分 类 号：O62[理学—有机化学]