Karlodinium elegans sp. nov. (Gymnodiniales, Dinophyceae), a novel species isolated from the East China Sea in a dinofl agellate bloom  

作　　者：Jingyi CEN Jianyan WANG Lifen HUANG Yarou LIN Guangmao DING Yuzao QI Songhui LÜ 

机构地区：[1]Research Center of Harmful Algae and Marine Biology,Jinan University,Guangzhou 510632,China [2]Southern Marine Science and Engineering Guangdong Laboratory,Zhuhai 519000,China [3]Department of Science Research,Beijing Museum of Natural History,Beijing 100050,China [4]Fujian Fishery Resources Monitoring Center,Fuzhou 350003,China [5]Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes,Jinan University,Guangzhou 510632,China

出　　处：《Journal of Oceanology and Limnology》2021年第1期242-258,共17页海洋湖沼学报（英文）

基　　金：Supported by the National Key R&D Program of China(No.2017YFC1404301);the National Natural Science Foundation of China(Nos.41906112,41606175);the Science&Technology Basic Resources Investigation Program of China(No.2018FY100200);the Beijing Natural Science Foundation(No.8194059)。

摘　　要：Abstract On May 24–29,2019,a harmful algal bloom occurred in Pingtan coastal areas,Fujian,southeast China,and caused mass mortality of cage-cultured fi sh.Two clonal cultures of an unknown naked dinofl agellate were set up from seawater samples taken during the bloom.The cultures were examined for morphological features,ultrastructure characters,photosynthetic pigments,the large subunit(LSU)of the rRNA gene,and the internal transcribed spacer(ITS)sequences,as well as acute toxicity analysis.The cell was unarmored,small-sized,and ovoid,and was characterized by elaborate striations on the epicone and hypocone.The nucleus was large,ellipsoid to oval or kidney-shaped,and centrally located in the cell.A long linear apical groove originated above the sulcus in the ventral epicone and extended to the dorsal side.An elongate,slit-like“ventral pore”was located on the left of the epicone,well away from the apical groove.The chloroplasts were yellowish brown,numerous,band like,and irregularly distributed in the cell periphery.Fucoxanthin was the main accessory pigment composition.Phylogeny topology reconstructed on partial LSU rDNA showed that the unknown dinofl agellate branched as a sister species to Karlodinium sp.(strain IFR981&IFR797,from France)and Karlodinium corrugatum(strain KDGSO08,from Australia),with genetic divergences of 0.6%and 3.3%,respectively.Based on the morphological and molecular phylogenetic analysis,we describe the novel dinofl agellate as Karlodinium elegans sp.nov.A toxicity assay revealed that the clonal culture of K.elegans(strain PTB601)had no adverse eff ect on brine shrimp(Artemia salina)and marine medaka(Oryzias melastigma),indicating it may not be a toxic species.

关 键 词：KARLODINIUM Kareniaceae harmful algal bloom TOXICITY dinofl agellate 

分 类 号：Q959[生物学—动物学]