Accelerated degradation of HAP/PLLA bone scaffold by PGA blending facilitates bioactivity and osteoconductivity  

作　　者：Cijun Shuai Wenjing Yang Pei Feng Shuping Peng Hao Pan 

机构地区：[1]State Key Laboratory of High Performance Complex Manufacturing,College of Mechanical and Electrical Engineering,Central South University,Changsha,410083,China [2]Institute of Bioadditive Manufacturing,Jiangxi University of Science and Technology,Nanchang,330013,China [3]Shenzhen Institute of Information Technology,Shenzhen,518172,China [4]NHC Key Laboratory of Carcinogenesis,School of Basic Medical Science,Central South University,Changsha,Hunan,410013,China [5]School of Energy and Machinery Engineering,Jiangxi University of Science and Technology,Nanchang,330013,China [6]Department of Periodontics&Oral Mucosal Section,Xiangya Stomatological Hospital,Central South University,Changsha,410013,China

出　　处：《Bioactive Materials》2021年第2期490-502,共13页生物活性材料（英文）

基　　金：This work was supported by the following funds:(1)The Natural Science Foundation of China(51905553,51935014,81871494,81871498);(2)Hunan Provincial Natural Science Foundation of China(2019JJ50774,2019JJ50588);(3)The Provincial Key R&D Projects of Jiangxi(20201BBE51012);(4)JiangXi Provincial Natural Science Foundation of China(20192ACB20005);(5)Guangdong Province Higher Vocational Colleges&Schools Pearl River Scholar Funded Scheme(2018);(6)The Project of Hunan Provincial Science and Technology Plan(2017RS3008);(7)The Project of State Key Laboratory of High Performance Complex Manufacturing,Central South University;(8)Shenzhen Science and Technology Plan Project(JCYJ20170817112445033).

摘　　要：The incorporation of hydroxyapatite(HAP)into poly-L-lactic acid(PLLA)matrix serving as bone scaffold is expected to exhibit bioactivity and osteoconductivity to those of the living bone.While too low degradation rate of HAP/PLLA scaffold hinders the activity because the embedded HAP in the PLLA matrix is difficult to contact and exchange ions with body fluid.In this study,biodegradable polymer poly(glycolic acid)(PGA)was blended into the HAP/PLLA scaffold fabricated by laser 3D printing to accelerate the degradation.The results indicated that the incorporation of PGA enhanced the degradation rate of scaffold as indicated by the weight loss increasing from 3.3%to 25.0%after immersion for 28 days,owing to the degradation of high hydrophilic PGA and the subsequent accelerated hydrolysis of PLLA chains.Moreover,a lot of pores produced by the degradation of the scaffold promoted the exposure of HAP from the matrix,which not only activated the deposition of bone like apatite on scaffold but also accelerated apatite growth.Cytocompatibility tests exhibited a good osteoblast adhesion,spreading and proliferation,suggesting the scaffold provided a suitable environment for cell cultivation.Furthermore,the scaffold displayed excellent bone defect repair capacity with the formation of abundant new bone tissue and blood vessel tissue,and both ends of defect region were bridged after 8 weeks of implantation.

关 键 词：PGA HAP/PLLA SCAFFOLD Degradation Bone regeneration 

分 类 号：R318[医药卫生—生物医学工程]