漆姑草醇提物对白血病细胞K562的诱导分化作用  

Induction of differentiation of K652 leukemia cells by Herba Saginae Japonicae

作　　者：伏琼[1] 唐文娟[1] 程勇[1] 贺仁政 梁冰[1,2] FU Qiong;TANG Wenjuan;CHENG Yong;HE Renzheng;LIANG Bing(Department of Pharmacology,Guizhou Medical University,Guiyang 550025;Key Laboratory of Environmental Pollution and Disease Monitoring,Ministry of Education,Guizhou Medical University,Guiyang 550025,Guizhou,China)

机构地区：[1]贵州医科大学药理学教研室,贵州贵阳550025 [2]贵州医科大学环境污染与疾病监控教育部重点实验室,贵州贵阳550025

出　　处：《癌变．畸变．突变》2021年第1期37-41,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：贵阳市科技计划项目(筑科合同[2017]30-9号);贵州省科技创新人才团队项目[黔科合平台人才(2016)5625号];贵州省2017年度“千”层次创新型人才项目。

摘　　要：目的:研究漆姑草醇提物(HSJ)对人慢性髓系白血病细胞(K562)的诱导分化作用。方法:实验设HSJ不同浓度(250、125、62.5μg/mL)实验组,阴性对照组(等体积1640培养基),作用48 h后,采用四甲基噻唑蓝(MTT)法检测K562细胞增殖抑制率;瑞氏-吉姆萨染色观察K562细胞形态;硝基四氮唑蓝(NBT)还原实验法测定K562细胞分化能力;流式细胞术检测K562细胞分化相关抗原CD11b、CD14、CD41a和CD42b阳性细胞的表达率;Western blot法检测K562细胞中珠蛋白转录因子1(GATA1)和造血转录因子1(PU.1)蛋白的表达。结果:与阴性对照组比较,各浓度HSJ均能有效抑制K562细胞的增殖(P<0.05);经HSJ作用后,各实验组K562细胞核浆比例减小,出现分叶状细胞核,部分细胞核凹陷出现明显的细胞分化形态;与阴性对照组相比,3个不同浓度HSJ组的NBT还原率均提高(P<0.05);CD11b、CD14、CD41a和CD42b阳性细胞表达率均增加(P<0.05);GATA1和PU.1蛋白相对含量亦增加,差异均具有统计学意义(P<0.05)。结论:漆姑草醇提物能诱导K562细胞向成熟细胞方向分化。OBJECTIVE:To study the effect of Herba Saginae Japonicae(HSJ)on differentiation of human chronic myeloid leukemia cells(K562).METHODS:K652 cells were organized into experimental groups(with treatment of 250,125,62.5μg/mL HSJ)and a negative control group.Cell proliferation was detected using the MTT assay.Cell morphology was observed after their staining by Wright-Giemsa.Their differentiation ability was determined by the NBT reduction assay.Their expression rates of CD11b,CD14,CD41a and CD42b positive cells were determined using flow cytometry.Western blot method was used to detect their expression of GATA1 and PU.1 proteins.RESULTS:Compared with the negative control group,HSJ exposure caused inhibition of proliferation of the K562 cells.Morphologically,treated cells showed lobulated nuclei and some showed obvious cell differentiation features.The different doses of treated cells showed significantly increased NBT reduction rates(P<0.05);CD11b,CD14,CD41a and CD42b positive rates(P<0.05)and GATA1 and PU.1 protein relative contents(P<0.05)compared with the negative controls(P<0.05).CONCLUSION:HSJ induced the K562 chronic myeloid leukemia cells to mature in the direction of differentiation.

关 键 词：漆姑草 K562细胞 诱导分化 白血病细胞 

分 类 号：R73-3[医药卫生—肿瘤]