Identification and molecular mechanism of angiotensin-converting enzyme inhibitory peptides from Larimichthys crocea titin  

作　　者：Yue Fan Zhipeng Yu Wenzhu Zhao Long Ding Fuping Zheng Jianrong Li Jingbo Liu 

机构地区：[1]College of Food Science and Engineering,Bohai University,Jinzhou,121013,China [2]Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University(BTBU),Beijing,102488,China [3]College of Food Science and Engineering,Northwest A&F University,Yangling,712100,China [4]Lab of Nutrition and Functional Food,Jilin University,Changchun,130062,China

出　　处：《Food Science and Human Wellness》2020年第3期257-263,共7页食品科学与人类健康（英文）

基　　金：supported by The National Natural Science Funds of China(No.31901635).

摘　　要：This study aimed to identify novel ACEI peptides from Larimichthys crocea titin using in silico approaches and to clarify the molecular interaction mechanism.The hydrolyzed peptides of titin were compared with known ACEI peptides in the AHTPDB and BIOPEP-UWM database.Furthermore,peptides were evaluated for their solubility,ADMET properties,ΔG(kcal/mol)values,and in vitro ACEI activity.Molecular mechanism of ACE-peptide was performed by molecular interactions and binding orientation study.The results revealed that IC50 values of Trp-Ala-Arg(WAR)and Trp-Gln-Arg(WQR)were(31.2±0.8)and(231.33±0.02)mol/L,respectively.The docking interactions result suggested that ACE-WAR and ACEWQR complexes have same binding site,including the residues LYS511,TYR520,TYR523,HIS353,and HIS513.Molecular docking of two tripeptides WAR and WQR with ACE studies predicted their binding site and clarified the interaction between ACE and its inhibitors.The molecular docking data are consistent with the ACE inhibitory activity of the studied peptides.The results showed that Larimichthys crocea titin may be a valuable source for developing nutraceutical food.

关 键 词：ACE peptide IDENTIFICATION In silico approaches ADMET Molecular docking 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]