Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran  

作　　者：Mohammad Mehdi Soltan Dallal Mohammad Reza Khoramizadeh Solmaz Agha Amiri Ali Akbar Saboor Yaraghi Ramin Mazaheri Nezhad Fard 

机构地区：[1]Division of Food Microbiology,Department of Pathobiology,School of Public Health,Tehran University of Medical Sciences,Tehran,Iran [2]Food Microbiology Research Center,Tehran University of Medical Sciences,Tehran,Iran [3]Department of Medical Biotechnology,School of Advanced Technologies in Medicine,Iran [4]Biosensor Research Center,Endocrinology and Metabolism Cellular and Molecular Science Institute,Tehran University of Medical Sciences,Iran [5]Division of Immunology,Department of Pathobiology,School of Public Health,Tehran University of Medical Sciences,Tehran,Iran

出　　处：《Food Science and Human Wellness》2016年第4期186-190,共5页食品科学与人类健康（英文）

摘　　要：Coagulase is considered as a major determinant factor for the identification of Staphylococcus aureus strains.The 3-end coding region of the coagulase(coa)gene contains a series of 81-bp tandem repeats,which differ in the number and location of enzymatic restriction sites among different isolates.coa PCR-RFLP has been used widely to type S.aureus isolates in epidemiological studies.The current study was conducted to investigate the coagulase gene polymorphisms in S.aureus isolated from various food samples using an in house PCR-RFLP method.A total of 100 strains of S.aureus were isolated from food samples.Isolates were typed by PCR-RFLP analysis using NdeI restriction digestion of the coagulase gene PCR products.Results showed that amplification of coagulase genes from S.aureus produced different PCR products.The isolates were grouped into 18 genotypes using RFLP analysis results of the genes.In this study,the S.aureus isolates have been shown to include more than one coagulase genotype,but only had a few coa genotypes predominated.

关 键 词：Staphylococcus aureus PCR-RFLP Coagulase gene Food samples 

分 类 号：TS201.3[轻工技术与工程—食品科学]