UV-NTG复合诱变选育高产糖化酶菌株  

Screening of High Glucoamylase Producing Strains with Compound Mutagenesis by UV and NTG

作　　者：王克芬 宋静静 王金余 王兴吉 刘胜利 刘文龙 Wang Kefen;Song Jingjing;Wang Jinyu;Wang Xingji;Liu Shengli;Liu Wenglong(Shandong Long Kete Enzyme Co.,ltd.,Shandong Province Key Laboratory of Enzyme Preparation Fermentation Technology,Linyi 276400,China)

机构地区：[1]山东隆科特酶制剂有限公司,山东省酶制剂发酵技术重点实验室,山东临沂276400

出　　处：《中国饲料添加剂》2020年第12期12-15,共4页China Feed Additive

摘　　要：以黑曲霉H-08为出发菌株,通过紫外线(UV)和亚硝基胍(NTG)复合诱变技术,筛选到1株酶活力提高明显的菌株UN3-36。经过多次复筛验证,证明其遗传性质稳定,酶活力显著提高,摇瓶的发酵酶活力可以达到19200 U/m L,较出发菌株提高了90%左右。The original strain of Aspergillus niger H-08 was modified in genetic characteristics with compound mutagenesis by UV and NTG.A high glucoamylase-yielding strain UN-36was screened from the mutants by slab screening and shake flask rescreening,which has high enzyme activity and good genetic stability.The enzyme activity of the shake flask-fermentationreached 19200 u/ml,which was 50%higher than that of the original strain.

关 键 词：糖化酶 UV NTG 复合诱变 筛选 

分 类 号：TS2[轻工技术与工程—食品科学与工程]