一株对茶丽纹象甲高毒力白僵菌菌株的筛选、鉴定与培养研究  

Screening,Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus

作　　者：王定锋[1] 李良德[1] 李慧玲[1] 张辉[1] 吴光远[1] WANG Dingfeng;LI Liangde;LI Huiling;ZHANG Hui;WU Guangyuan(Tea Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)

机构地区：[1]福建省农业科学院茶叶研究所,福建福州350013

出　　处：《茶叶科学》2021年第1期101-112,共12页Journal of Tea Science

基　　金：福建省公益类科研院所基本科研专项(2019R1029-4);福建省农业科学院茶叶研究所重点项目(2014-cys-02);国家茶叶产业技术体系咀嚼式害虫防控岗位(CARS-19)。

摘　　要：茶丽纹象甲(Myllocerinus aurolineatus)是一种重要的茶树害虫,当前对其防治仍以化学手段为主。为寻找一种高效、安全和可持续的生物防治方法,本研究采用浸虫法从12株原寄主为鞘翅目昆虫的白僵菌Beauveria spp.中筛选出一株对茶丽纹象甲成虫高毒力的Bbr1552菌株。室内生物测定结果表明,用每毫升含5.0×107个孢子的孢悬液(25℃)处理茶丽纹象甲成虫的LT50值为4.49 d,处理7 d,茶丽纹象甲的累计校正死亡率为100%,僵虫率为69.44%,LC50值为每毫升1.55×106个孢子。对菌株Bbr1552进行形态学鉴定与rDNA-ITS序列和Bloc序列分析,明确其为布氏白僵菌Beauveria brongniartii。此外,对该菌株的最适培养基和培养温度研究发现,其最适生长和产孢培养基均为PPDA培养基;最适生长和产孢温度均为25℃。Myllocerinus aurolineatus(Coleoptera:Curculionidae)is one of the most important pests in tea plantation,and chemical control is the usual treatment method for this weevil at present.In order to explore alternative strategy for sustainable control of the weevil,dip method was employed to test the virulence of 12 isolates of Beauveria sp.,which isolated from insects of Coleoptera.When inoculated with spore suspension(5.0×107 spores·mL-1),the strain Bbr1552 showed the highest virulence against the adults of M.aurolineatus,which caused the highest corrected mortality rates of 100%,shortest LT50 of 4.49 d and highest cadaver rates of 69.44%.The LC50 value was 1.55×106 spores·mL-1 on 7 d(25℃)after inoculation with Bbr1552.According to morphological identification and analysis of rDNA-ITS and Bloc sequences,the strain Bbr1552 was identified to be a B.brongniartii.Besides,the PPDA medium and 25℃were the most optimal conditions for the colony growth and sporulation of the strain Bbr1552.

关 键 词：茶丽纹象甲 布氏白僵菌 毒力 鉴定 培养条件 

分 类 号：S571.1[农业科学—茶叶生产加工] S435.711[农业科学—作物学]