环状RNA hsa_circ_0026344对乳腺癌细胞增殖和侵袭的相关研究  

Circular RNA hsa_circ_0026344 on the Proliferation and Invasion of Breast Cancer Cells

作　　者：党少华[1] 徐敏 胡金月[1] 孙永强[1] DANG Shaohua;XU Min;HU Jinyue;SUN Yongqiang(Department of Breast and Thyroid Surgery,the Third People’s Hospital of Zhengzhou,Zhengzhou 450000,China;Department of Endocrinology,the Third People’s Hospital of Zhengzhou,Zhengzhou 450000,China)

机构地区：[1]郑州市第三人民医院乳腺甲状腺外科,河南郑州450000 [2]郑州市第三人民医院内分泌科,河南郑州450000

出　　处：《河南医学研究》2021年第2期193-196,共4页Henan Medical Research

基　　金：河南省郑州市科技攻关项目(20150037)。

摘　　要：目的探讨hsa_circ_0026344在乳腺癌进展中的作用。方法采用qRT-PCR检测hsa_circ_0026344在乳腺癌组织中的表达,利用慢病毒构建稳定转染hsa_circ_0026344过表达细胞模型,CCK-8检测细胞增殖,Transwell小室检测细胞侵袭能力,流式细胞仪检测细胞凋亡,明确hsa_circ_0026344在乳腺癌细胞恶性生物学行为中的作用。结果hsa_circ_0026344在乳腺癌组织及细胞中表达降低,并且与不良预后相关。hsa_circ_0026344过表达能够抑制乳腺癌细胞的增殖能力、侵袭能力,促进细胞凋亡。结论hsa_circ_0026344可能为乳腺癌潜在的生物学标志物,参与调控乳腺癌的进展。Objective To investigate the role of hsa_circ_0026344 in breast cancer progression.Methods The expression of hsa_circ_0026344 in breast cancer was detected by qRT-PCR.Stable transfection of hsa_circ_0026344 overexpressed cells was established by lentivirus,cck-8 was used to detect cell proliferation,and Transwell was used to detect cell invasion,and flow cytometry was used to detect apoptosis.To determine the role of hsa_circ_0026344 in malignant biological behavior of breast cancer cells.Results hsa_circ_0026344 was decreased in breast cancer tissues and cells,and was associated with poor prognosis.hsa_circ_0026344 overexpression can significantly inhibit the proliferation and invasion ability of breast cancer cells,and promote apoptosis.Conclusion hsa_circ_0026344 may be a potential biological marker of breast cancer,which is involved in regulating the progress of breast cancer.

关 键 词：乳腺癌 环状RNA hsa_circ_0026344 增殖 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]