利用GFP标记的Ralstonia solanacearum鉴定马铃薯青枯病抗性  

Identification of potato bacterial wilt resistance using GFP-labeled Ralstonia solanacearum

作　　者：陈卓 肖熙鸥[2] 陈曙[2] 李可[2] 邹华芬 金辉 CHEN Zhuo;XIAO Xiou;CHEN Shu;LI Ke;ZOU Huafen;JIN Hui(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163000,Heilongjiang,China;Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs/Key Laboratory for Post-harvest Physiology and Technology of Tropical Horticultural Products of Hainan Province/Institute of South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Sciences Zhanjiang 524091,Guangdong,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163000 [2]农业农村部热带果树生物学重点实验室·海南省热带园艺产品采后生理与保鲜重点实验室·中国热带农业科学院南亚热带作物研究所,广东湛江524091

出　　处：《中国瓜菜》2021年第1期35-41,共7页China Cucurbits And Vegetables

基　　金：中国热带农业科学院基本科研业务费专项基金项目(1630062017016);广东省甘薯马铃薯产业技术体系创新团队项目(2019KJ111);广东省现代种业提升项目(粤财农[2019]73号)。

摘　　要：青枯病是危害马铃薯产业的重要病害,选育与推广抗青枯病品种是防控青枯病最高效的手段。目前已有的人工接种和田间抗性鉴定的方法不能有效区分处于潜伏侵染与完全抵抗侵染的材料,而利用GFP标记的Ralstonia solanacearum能够有效解决上述问题。笔者通过电击转化法将广宿主载体pBBR1MCS2-Tac-EGFP导入青枯菌P2中,成功获得了能够稳定遗传且不影响其致病力并带有绿色荧光报告的青枯菌菌株P2-Tac-EGFP。通过对感抗材料进行接种,结果表明,P2-Tac-EGFP能够有效区分感抗材料,并且P2-Tac-EGFP在感病材料的根部和茎部大量分布,而在抗病材料的根部仅有少量分布。综上所述,利用GFP标记的R.solanacearum能够快速准确地鉴定马铃薯青枯病抗性。Bacterial wilt is an important disease that endangers the potato industry.The selection and promotion of bacterial wilt resistant varieties is the most effective means to prevent and control bacterial wilt.The selection of germplasm resistant to bacterial wilt is the basis for the selection of resistant varieties.The current methods of artificial inoculation and field resistance identification cannot effectively distinguish between latent infection and disease-resistant materials,and Ralstonia solanacearum labeled with GFP can effectively solve the above problems.In this study,the broad host vector pBBR1MCS2-Tac-EGFP was introduced into R.solanacearum P2 by the electric shock transformation method,and successfully obtained the R.solanacearum strain P2-Tac-EGFP with stable inheritance without affecting its pathogenicity.Through inoculation of resistant materials,the results show that P2-Tac-EGFP can effectively distinguish the resistant materials,and P2-Tac-EGFP is distributed in a large amount in the roots and stems of the disease-resistant materials,while a small amount is in the roots of the resistant materials.In summary,the use of GFP-labeled R.solanacearum can quickly and accurately identify potato bacterial wilt resistance.

关 键 词：马铃薯 Ralstonia solanacearum GFP 抗性 

分 类 号：S532[农业科学—作物学]