ApoE^-/-小鼠三维DANTE-FLASH颈动脉血管壁成像:基于7.0T磁共振技术  

Carotid artery wall imaging using 7.0T magnetic resonance imaging with threedimensional DANTE-prepared FLASH in ApoE^-/-mice

作　　者：杨渊博 李忠豪 刘蔷 谢国喜 冯衍秋[1,2] YANG Yuanbo;LI Zhonghao;LIU Qiang;XIE Guoxi;FENG Yanqiu(School of Biomedical Engineering,Southern Medical University,Guangzhou,510515,China;Guangdong Provincial Key Laboratory of Medical Image Processing,Southern Medical University,Guangzhou 510515,China;Department of Pathophysiology//Key Lab for Shock and Microcirculation Research of Guangdong Province//School of Basic Medical Sciences,Southern Medical University,Guangzhou,510515,China;Department of Biomedical Engineering,Sixth Affiliated Hospital,School of Basic Sciences,Guangzhou Medical University,Guangzhou 511436,China)

机构地区：[1]南方医科大学生物医学工程学院,广东广州510515 [2]南方医科大学广东省医学图像处理重点实验室,广东广州510515 [3]南方医科大学基础医学院//广东省病理生理教研室//广东省休克与微循环研究重点实验室,广东广州510515 [4]广州医科大学基础学院,第六附属医院,生物医学工程系,广东广州511436

出　　处：《南方医科大学学报》2021年第2期216-222,共7页Journal of Southern Medical University

基　　金：国家自然科学基金(81871349);广东省科技计划项目(2018B030333001)。

摘　　要：目的探讨7.0 T磁共振上三维血管壁成像技术(DANTE-FLASH)进行ApoE-/-小鼠颈动脉粥样硬化斑块成像的可行性。方法通过数值仿真优化成像参数,实现血管腔-壁对比度最大化。6只ApoE-/-小鼠和3只野生型小鼠在7.0 T MRI均行DANTEFLASH和流入流出饱和带的多层2D RARE(2D-IOSB-RARE)两种扫描。在管壁信噪比(SNRwall)、管腔信噪比(SNRlumen)、壁-腔对比噪声比(CNR)、管腔面积(LA)、和管壁面积(WA)方面分别对2D-IOSB-RARE和DANTE-FLASH序列进行比较。对于LA与WA,使用线性回归分析评估MR图像与病理图像测量结果的相关性。结果根据数值仿真结果,小鼠实验的DANTE模块采用了15°翻转角与150脉冲链。相较于2D-IOSB-RARE,DANTE-FLASH的CNR轻微降低(P<0.001),但层分辨率显著提高。组内相关系数(LA:ICC=0.94,P<0.001;WA:ICC=0.93,P<0.001)和Bland-Altman图显示出DANTE-FLASH和2DIOSB-RARE在评估LA和WA测量结果方面具有良好的一致性。MR图像与病理图像在定量测量LA(P<0.0001)和WA(P<0.0001)中呈现较强相关性。结论在7.0 T磁共振上,三维DANTE-FLASH黑血MR序列,可实现各向同性高空间分辨率成像,有利于ApoE-/-小鼠颈动脉粥样硬化病变的灵活显示和定量评估。Objective To explore the feasibility of three-dimensional(3D)vessel wall imaging of carotid atherosclerotic plaques in ApoE-/-mice using 7.0T magnetic resonance imaging(MRI)with delays alternating with nutations for tailored excitation(DANTE)-prepared fast low-angle shot(DANTE-FLASH)technique.Methods Numerical simulations were performed for optimizing imaging parameters to maximize the wall-lumen contrast.Six ApoE-/-and three wild-type mice were scanned using a 7.0T MRI scanner with DANTE-FLASH and multi-slice 2D RARE coupled with inflow outflow saturation bands(2D-IOSBRARE).The wall signal-to-noise ratio(SNRwall),lumen SNR(SNRlumen),wall-lumen contrast-to-noise ratio(CNR),lumen area(LA),and wall area(WA)were compared between DANTE-FLASH and 2D-IOSB-RARE sequences.Linear regression analysis was performed to assess the correlation between the MRI measurements and histopathological measurements of LA and WA.Results Based on the simulation results,a flip angle of 15°and a train length of 150 were implemented in the live imaging study.Compared with 2D-IOSB-RARE,DANTE-FLASH provided a slightly reduced CNR(P<0.001)but much improved slice resolution.The LA and WA measurements from the DANTE-FLASH and 2D-IOSB-RARE showed excellent agreement based on ICC analysis(LA:ICC=0.94,P<0.001;WA:ICC=0.93,P<0.001)and Bland-Altman plots.Strong correlations were observed between the MRI and histopathological measurements for both LA(P<0.0001)and WA(P<0.0001).Conclusion As a 3D black-blood MR sequence,DANTE-FLASH provides isotropic high spatial resolution to allow reliable visualization and quantitative evaluation of the arteriosclerotic lesions within the carotid artery of ApoE-/-mice using a 7.0T MRI scanner.

关 键 词：动脉粥样硬化 黑血 颈动脉血管壁成像 APOE^-/-小鼠 DANTE 3D FLASH 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R445.2[医药卫生—临床医学]