PARP-1抑制剂PJ34对前列腺癌细胞生物学行为的影响  

Effects of poly(ADP-ribose)polymerase-1 inhibitor PJ34 on biological behaviors of prostate cancer cell

作　　者：高清河 李金雨 赵力 于忠英 杨镇有 庄园丽 Gao Qinghe;Li Jinyu;Zhao Li;Yu Zhongying;Yang Zhenyou;Zhuang Yuanli(Department of Urinary Surgery,The No.909 Hospital,Joint Logistic Support Force of PLA)

机构地区：[1]中国解放军联勤保障部队第九〇九医院泌尿外科,漳州363000

出　　处：《重庆医科大学学报》2021年第1期65-68,共4页Journal of Chongqing Medical University

基　　金：中国解放军联勤保障部队第九○九医院青年苗圃基金资助项目(编号:20YQ003)。

摘　　要：目的:观察聚腺苷二磷酸核糖聚合酶-1[poly(ADP-ribose) polymerase-1,PARP-1]抑制剂PJ34对前列腺癌PC-3细胞增殖、凋亡、迁移、侵袭等生物学行为的影响。方法:使用不同浓度PJ34处理PC-3细胞后,CCK-8法检测细胞增殖,Western blot检测PARP-1蛋白表达,annexin V-FITC/IP双染法检测细胞凋亡,Transwell法检测细胞迁移、侵袭等相关生物学行为的变化。结果:PJ34组分别作用72 h、48 h、24 h后,PARP-1蛋白水平均明显低于control组(t=4.647,P=0.009 7;t=4.816,P=0.008 5;t=17.28,P<0.000 1)。PJ34组凋亡率为(17.64±4.49)%,与control组凋亡率的(3.83±0.74)%相比,差异有统计学意义(t=5.261,P=0.006)。Control组和PJ34组细胞迁移差异有统计学意义(t=23.67,P<0.000 1),细胞侵袭差异有统计学意义(t=29.31,P<0.000 1)。结论:PJ34通过抑制PARP-1的表达抑制前列腺癌细胞PC-3的增殖、迁移和侵袭能力。Objective:To investigate the effect of PJ34,poly(ADP-ribose)polymerase-1(PARP-1)inhibitor,on the biological behavior of human prostate cancer cell line PC-3,including cells proliferation,apoptosis,migration and invasion.Methods:PJ34 with different concentration was used to treat PC-3 cells,CCK-8 assay was used to detect cell proloferation,Western blot was used to detect the expression of PARP-1,annexin V-FITC/IP double-staining method was used to detect cell apoptosis,Transwell method was used to detect changes of cell biological behavior including migration and invasion.Results:After treatment for 72 h,48 h and 24 h,PRAP-1 levels in the PJ34 group were lower than those in the control group(t=4.647,P=0.0097;t=4.816,P=0.0085;t=17.28,P<0.0001).Apoptosis rate in the PJ34 group was(17.64±4.49)%,while in the control group was(3.83±0.74)%,with statistically significant differences(t=5.261,P=0.006).Differences in migration had statistical significance in two groups(t=23.67,P<0.0001).In addition,cell invasion had statistically significant differences(t=29.31,P<0.0001).Conclusion:PJ34 can inhibit PC-3 cells’proliferation,apoptosis and invasion by inhibiting expression of PRAP-1.

关 键 词：前列腺癌 聚腺苷二磷酸核糖聚合酶-1 生物行为学 迁移 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]