结缔组织生长因子促进人卵巢癌细胞系SKOV3增殖与迁移  

Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3

作　　者：董玲玲[1] 高云 牛国宇 DONG Ling-ling;GAO Yun;NIU Guo-yu(Tumor Center,Weifang Hospital of Traditional Chinese Medicine;School of Public Health and Management,Weifang Medical University,Weifang 261000,China)

机构地区：[1]潍坊市中医院肿瘤中心 [2]潍坊医学院公共卫生与管理学院,山东潍坊261000

出　　处：《基础医学与临床》2021年第2期193-196,共4页Basic and Clinical Medicine

基　　金：潍坊市科技发展计划项目(2019YX015)。

摘　　要：目的探讨结缔组织生长因子(CTGF)对人卵巢癌细胞系SKOV3增殖、迁移能力的影响,并探索其可能机制。方法用重组人CTGF(rhCTGF)处理SKOV3细胞;利用CCK-8法和集落形成实验检测SKOV3细胞的增殖;利用细胞划痕实验检测SKOV3细胞的迁移;Western blot检测上皮-间充质转化(EMT)相关蛋白表达。结果rhCTGF处理后,SKOV3细胞的增殖率增高,集落形成、细胞迁移能力增强(P<0.05)。EMT相关蛋白E-cadherin表达降低,而Vimentin表达增高(P<0.05)。结论CTGF能够促进SKOV3细胞的增殖及迁移,该作用可能与其调控EMT有关。Objective To investigate the effects of CTGF on proliferation and migration of SKOV3 and the underlying mechanism.Methods SKOV3 cells were treated with recombinant human CTGF(rhCTGF).The proliferation of SKOV3 was detected by CCK-8 assay and colony formation assay.The migration of SKOV3 was detected by wound healing assay.The expression of EMT-related proteins was detected by Western blot.Results After rhCTGF treatment,the proliferation rate of SKOV3 was increased,the colony formation and migzation were enhanced(P<0.05).The expression of E-cadherin was decreased and the expression of vimentin was increased,which were related proteins of EMT(P<0.05).Conclusions CTGF can promote proliferation,growth and metastasis of SKOV3,and that may contribute to the regulation of epithelium-mesenchymal transformation(EMT).

关 键 词：结缔组织生长因子 卵巢癌细胞 增殖 迁移 机制 

分 类 号：R737.31[医药卫生—肿瘤]