长链非编码RNA MCM3AP-AS1靶向调控miR-876-5p促进人结直肠癌细胞系的增殖和迁移  

Long non-coding RNA MCM3AP-AS1 targeted miR-876-5p promotes the proliferation and migration of colorectal cancer cell lines

作　　者：周堤侠 吕海栋[1] ZHOU Di-xia;LYU Hai-dong(Department of Tumor Surgery,Qinghai Provincial People's Hospital,Xining 810007,China)

机构地区：[1]青海省人民医院肿瘤外科,青海西宁810007

出　　处：《基础医学与临床》2021年第2期225-232,共8页Basic and Clinical Medicine

摘　　要：目的探讨长链非编码RNA(lncRNA)微小染色体维持蛋白3相关蛋白-反义链1(MCM3AP-AS1)靶向调控miR-876-5p对结直肠癌(CRC)细胞的增殖和迁移的作用。方法实时定量聚合酶链式反应法(RT-qPCR)检测CRC组织和细胞中MCM3AP-AS1的表达;用MTT法、划痕愈合实验和Transwell小室法检测CRC细胞的增殖和迁移;采用生物信息分析、双荧光素酶基因报告实验、RT-qPCR验证MCM3AP-AS1和miR-876-5p的靶向关系。结果与非肿瘤组织和正常结直肠上皮细胞系相比,MCM3AP-AS1在CRC组织和细胞系中表达上调(P<0.05);敲低MCM3AP-AS1能抑制CRC细胞增殖和迁移(P<0.05);生物信息学分析、双荧光素酶基因报告实验和RT-qPCR表明MCM3AP-AS1与miR-876-5p可相互作用;miR-876-5p可削弱MCM3AP-AS1对CRC细胞增殖和迁移的促进作用(P<0.05)。结论MCM3AP-AS1可以通过吸附miR-876-5p促进CRC细胞的增殖和迁移。Objective To investigate the effect and mechanism of long non-coding RNA MCM3AP antisense transcript 1 and miR-876-5p on the proliferation and migration of colorectal cancer(CRC)cells in vitro.Methods The expressions of MCM3AP-AS1 in CRC tissue and cells were detected by real time quantitative polymerase chain reaction(RT-qPCR).The proliferation and migration of CRC cells were detected by MTT,scratch test and Transwell assay;and the targeting relationship between MCM3AP-AS1 and miR-876-5p was verified by bioinformatics analysis,luciferase assay and RT-qPCR.Results Compared with normal adjacent tissues and normal CRC epithelial cell lines,the expression of MCM3AP-AS1 was up-regulated.Down-regulation of MCM3AP-AS1 decreased the proliferation and migration of colorectal cancer cells.MCM3AP-AS1 targeted at miR-876-5p and down-regulated its expression.miR-876-5p inhibited the promotion of malignant phenotype of colon cancer.Conclusions MCM3AP-AS1 may promote the proliferation and migration of CRC cancer cells by absorbing of miR-876-5p.

关 键 词：MCM3AP-AS1 miR-876-5p 结直肠癌 增殖 迁移 

分 类 号：R735[医药卫生—肿瘤]