MiR-371a-5p表达与非小细胞肺癌预后的关系  

ASSOCIATION OF MICRORNA-371A-5P EXPRESSION WITH THE PROGNOSIS OF NON-SMALL CELL LUNG CANCER

作　　者：崔耀杰 金翔凤[1] 刘玉洪[1] CUI Yaojie;JIN Xiangfeng;LIU Yuhong(Department of Thoracic Surgery,The Affiliated Hospital of Qingdao University,Qingdao 266003,China)

机构地区：[1]青岛大学附属医院胸外科,山东青岛266003

出　　处：《青岛大学学报（医学版）》2021年第1期69-72,共4页Journal of Qingdao University(Medical Sciences)

基　　金：山东省医药卫生科技发展计划项目(2018WS383)。

摘　　要：目的探讨微小RNA-371a-5p(miR-371a-5p)在非小细胞肺癌(NSCLC)中的表达及临床意义。方法采用实时定量聚合酶链反应技术(RT-qPCR)检测136例NSCLC肿瘤组织和其癌旁正常组织(距离肿瘤5 cm以上)标本miR-371a-5p的表达,应用χ^2检验分析miR-371a-5p表达与病人临床病理特征之间关系,Kaplan-Meier生存分析模型分析miR-371a-5p表达与NSCLC病人总生存期及无病生存期的相关性。采用单因素和多因素Cox模型分析miR-371a-5p对NSCLC病人预后预测价值。结果与癌旁正常组织相比,NSCLC肿瘤组织miR-371a-5p表达明显升高(t=12.515,P<0.01)。NSCLC病人miR-371a-5p高表达与肿瘤大小(χ^2=4.960,P<0.05)、TNM分期(χ^2=7.061,P<0.05)和淋巴结转移(χ^2=10.675,P<0.01)显著相关。Kaplan-Meier生存分析显示,miR-371a-5p高表达组病人的总生存期低于miR-371a-5p低表达组(χ^2=9.051,P<0.001)。单因素和多因素Cox分析表明,miR-371a-5p表达可作为NSCLC病人总生存期的独立预测指标(HR=1.8,95%CI=1.0~3.2,P<0.05)。结论MiR-371a-5p在NSCLC肿瘤组织中高表达并可以作为病人的预后生物标志物。Objective To investigate the expression and clinical significance of microRNA-371a-5p(miR-371a-5p)in non-small cell lung cancer(NSCLC).Methods Real-time quantitative PCR was used to measure the expression of miR-371a-5p in 136 NSCLC tissue samples and corresponding adjacent tissue samples(more than 5 cm away from the tumor).The chi-square test was used to investigate the association between miR-371a-5p expression and clinical features,and the Kaplan-Meier survival analysis was used to analyze the correlation of miR-371a-5p expression with overall survival and disease-free survival of NSCLC patients.Univariate and multivariate Cox analyses were used to determine the value of miR-371a-5p in predicting the prognosis of NSCLC patients.Results Compared with the adjacent tissue samples,NSCLC tissue samples had a significant increase in the expression of miR-371a-5p(t=12.515,P<0.01).The high expression of miR-371a-5p in NSCLC patients was significantly asso-ciated with tumor size(χ^2=4.960,P<0.05),TNM stage(χ^2=7.061,P<0.05),and lymph node metastasis(χ^2=10.675,P<0.01).The Kaplan-Meier survival analysis showed that the patients with high expression of miR-371a-5p had significantly lower overall survival than those with low expression of miR-371a-5p(χ^2=9.051,P<0.01).The univariate and multivariate Cox analyses showed that the expression of miR-371a-5p(HR=1.8,95%CI=1.0-3.2,P<0.05)could be used as an independent predictive factor for the overall survival of patients with NSCLC.Conclusion MiR-371a-5p is highly expressed in NSCLC tissue and can be used as a prognostic biomarker for NSCLC patients.

关 键 词：癌 非小细胞肺 微RNAS 预后 

分 类 号：R734.2[医药卫生—肿瘤]