分化型甲状腺癌组织中浸润免疫细胞的类型及其与临床特征的关系  

The relationship between immune infiltrating cells and clinicopathological characteristics in differentiated thyroid carcinoma

作　　者：徐佩 关海霞[1] XU Pei;GUAN Haixia(Department of Endocrinology and Metabolism,the Endocrine Institute and the Liaoning Provincial Key Laboratory of Endocrine Diseases,the First Hospital of China Medical University,Liaoning Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院内分泌科,内分泌研究所,辽宁省内分泌疾病重点实验室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2021年第3期397-402,共6页Journal of Modern Oncology

基　　金：辽宁省重点研发计划指导计划(编号:2017225008);辽宁省“百千万人才工程”培养经费资助项目—沈阳市中青年科技创新人才支持计划(编号:RC170058)。

摘　　要：目的:探讨分化型甲状腺癌(DTC)组织中浸润免疫细胞的类型,分析其与肿瘤临床病理特征和风险分期的关系。方法:利用TCGA数据库中转录本数据,通过CIBOSORT反卷积法计算22种免疫细胞在DTC组织中的浸润情况,比较DTC组织与正常甲状腺组织之间的免疫细胞差异,并对DTC按照不同临床病理特征和不同肿瘤分期进行分组,比较组间浸润免疫细胞的差异。结果:DTC组织的浸润免疫细胞中,M0巨噬细胞、M2巨噬细胞、静息态树突状细胞、未活化的肥大细胞浸润丰度明显高于正常甲状腺组织。CD8~+T细胞和浆细胞在DTC组织中的浸润相对丰度,AJCCⅢ/Ⅳ期患者低于AJCCⅠ/Ⅱ期(P<0.05),而单核细胞、活化的肥大细胞和静息态树突状细胞在DTC组织浸润相对丰度则随着AJCC分期增高而增加(P<0.05)。结论:DTC组织中浸润免疫细胞的优势类型与肿瘤恶性程度相关,提示免疫细胞微环境可能参与肿瘤进展,并可作为评估DTC风险度的辅助指标。Objective:To explore immune infiltrating cells distribution in differentiated thyroid carcinoma(DTC)and to study the relationship between cells and clinical features.Methods:Transcripts and cilinical data were downloaded from TCGA calculate 22 types of immune cells infiltrating in thyroid by using CIBOSORT,and different immune infiltrating cells were compared between DTC and normal thyroid,and association was andysed between cells and different tumor staging.Results:M0 macrophages,M2 macrophages,resting dendritic cells and resting mast cells in DTC were significantly higher than those in normal thyroid.Furthermore,CD8+T cells and plasma cells in AJCC stageⅢ/Ⅳpatients were lower than in AJCC stageⅠ/Ⅱpatients(P<0.05),while the abundance of monocytes,activated mast cells and resting dendritic cells in DTC increased with AJCC stage(P<0.05).Conclusion:The predominant types of immune infiltrating cells in thyroid are related to tumor progression,indicating that immune cell existing in microenvironment may prompt tumor progression,and certain immune cell types may assess tumor risks.

关 键 词：分化型甲状腺癌 免疫微环境 免疫细胞 浸润 临床特征 肿瘤分期 

分 类 号：R736.1[医药卫生—肿瘤]