结肠腺癌组织中Keap1的表达及其与Ki67增殖指数的相关性  

Expression of Keap1 and relationship with Ki67 index in colorectal adenocarcinoma tissues

作　　者：孙德俊 孔永和 SUN Dejun;KONG Yonghe(Surgery Staff Room,Department of Clinical Medicine,Qinghai Institute of Heath Sciences,Qinghai Xining 810000,China;Department of Radiotherapy,the Fifth People's Hospital of Qinghai Province(Cancer Hospital),Qinghai Xining 810000,China)

机构地区：[1]青海卫生职业技术学院临床医学系外科教研室,青海西宁810000 [2]青海省第五人民医院(肿瘤医院)放疗科,青海西宁810000

出　　处：《现代肿瘤医学》2021年第3期458-462,共5页Journal of Modern Oncology

摘　　要：目的:检测结肠腺癌组织中Kelch样环氧氯丙胺相关蛋白1(Keap1)的表达,分析其与Ki67增殖指数(Ki67)的相关性。方法:本实验以93例结肠腺癌术后的肿瘤组织作为观察组,选择距肿物边缘>3 cm经病理证实的正常结肠黏膜组织作为对照组。应用免疫组化法和荧光PCR法检测两组中Keap1蛋白和mRNA的表达。应用相关分析法分析Keap1与Ki67增殖指数的相关性。结果:免疫组化方法显示两组中Keap1蛋白的阳性率差异有统计学意义。观察组中Keap1的阳性率与肿物最大径、淋巴结转移、脉管癌栓、浸润深度和TNM分期相关,而与性别、年龄、分化程度、是否有癌结节无关。观察组中Keap1的表达与预后相关。观察组中Keap1的表达与Ki67增殖指数呈负相关。荧光PCR法显示两组中Keap1 mRNA的表达差异有统计学意义。结论:Keap1在结肠腺癌组织中低表达,可能与更大的肿瘤直径、更多的淋巴结转移、脉管癌栓的出现、更深的肿瘤浸润及更晚的TNM分期相关。Keap1可能通过抑制细胞增殖起作用,术后检测Keap1的表达对判断预后有一定价值。Objective:To detect expression of Keap1 in colorectal adenocarcinoma tissues,analyze its relationship with Ki67 index.Methods:93 cases of colorectal adenocarcinoma were selected as observation group,and normal colorectal mucosa tissues(>3 cm from tumor edge)were selected as control group.Expression of Keap1 was detected by IHC and fluorescence PCR in two groups.Relationship between Keap1 and Ki67 proliferation index was analyzed in observation group.Results:IHC results showed that expression of Keap1 was significantly different in two groups.Positive rate of Keap1 was related to tumor maximum diameters,lymph node metastasis,vascular tumor thrombus,infiltration depth and TNM staging of observation groups.Positive rate of Keap1 was not related to gender,age,differentiation degree and cancer nodules.The expression of Keap1 was related to prognosis in observation group.Expression of Keap1 had a negative correlation with Ki67 proliferation index in observation group.Expression of Keap1 mRNA was statistically significant in two groups.Conclusion:The low expression of Keap1 may be associated with larger tumor diameter,more lymph node metastasis,presence of vascular cancer thrombus,deeper tumor infiltration and later TNM staging.Keap1 may play a role in inhibiting cell proliferation.Expression of Keap1 has certain value in judging prognosis in colorectal adenocarcinoma.

关 键 词：结肠腺癌 KEAP1 KI67 预后 

分 类 号：R735.35[医药卫生—肿瘤]