miR-10a-5p靶向ITSN1对宫颈癌细胞增殖和顺铂化疗敏感性的影响  

Effect of miR-10a-5p targeting ITSN1 on cervical cancer cell proliferation and cisplatin chemotherapy sensitivity

作　　者：李妘[1] 王静 LI Yun;WANG Jing(Department of Gynecology,Hanzhong Central Hospital,Hanzhong 723000,China;Department of Gynecological Oncology,Shaanxi Cancer Hospital)

机构地区：[1]陕西省汉中市中心医院妇科,汉中723000 [2]陕西省肿瘤医院妇瘤科

出　　处：《山西医科大学学报》2021年第1期32-37,共6页Journal of Shanxi Medical University

摘　　要：目的探讨miR-10a-5p对宫颈癌细胞增殖及对化疗药物顺铂敏感性的影响及机制。方法实时荧光定量PCR(qRT-PCR)检测23对宫颈癌及配对癌旁组织miR-10a-5p的相对表达水平。利用空载体病毒(阴性对照组)或miR-10a-5p过表达慢病毒(miR-10a-5p过表达组)感染人宫颈癌细胞株Caski,同时设置不作处理的空白对照组。使用CCK-8法检测各组细胞增殖及对顺铂敏感性的影响,检测指标为吸光度和细胞生长抑制率。qRT-PCR和Western blot检测三组细胞中ITSN1的mRNA和蛋白水平。使用荧光素酶报告基因验证miR-10a-5p的潜在靶点。结果与宫颈癌癌旁组织相比,宫颈癌组织中miR-10a-5p表达显著降低(P<0.05)。与空白对照组及阴性对照组相比,miR-10a-5p过表达组细胞吸光度显著减小,细胞增殖能力显著降低(P<0.05),对顺铂的化疗敏感性显著增强(P<0.05),空白对照组和阴性对照组间细胞增殖能力和对顺铂的化疗敏感性差异无统计学意义(P>0.05)。miR-10a-5p可与ITSN1靶向结合,降低ITSN1表达水平。结论miR-10a-5p可能通过靶向ITSN1抑制Caski细胞增殖,并增强Caski细胞对顺铂的药物敏感性。Objective To investigate the effect of miR-10a-5p on the proliferation and the sensitivity of cervical cancer cells to cisplatin and its mechanism.Methods The relative expression level of miR-10a-5p in cervical cancer and paracancerous tissues was detected by real-time fluorescence quantitative PCR(qRT-PCR).Empty vector lentivirus(negative control group)or miR-10a-5p overexpression lentivirus(miR-10a-5p overexpression group)were used to infect the human cervical cancer cell line Caski.At the same time,a blank control group without any treatment was set up.CCK-8 method was used to detect the cell proliferation and its effect on the sensitivity of cisplatin,and the detection indicators were absorbance and cell growth inhibition rate.The mRNA and protein levels of ITSN1 in the three groups were detected by qRT-PCR and Western blot,and the potential targets of miR-10a-5p were verified by luciferase reporter gene.Results Compared with the adjacent tissues of cervical cancer,the expression of miR-10a-5p in cervical cancer tissue was significantly decreased(P<0.05).Compared with blank control group and negative control group,the cell absorbance was significantly decreased in miR-10a-5p overexpression group,the proliferation ability was significantly decreased and the chemosensitivity to cisplatin was significantly enhanced(all P<0.05).There was no statistically significant difference in cell proliferation ability and chemotherapy sensitivity to cisplatin between blank control group and negative control group(P>0.05).MiR-10a-5p was the target gene of ITSN1,and down-regulated the expression of ITSN1.Conclusion The miR-10a-5p may inhibit the proliferation of Caski cells and enhance the drug sensitivity of Caski cells to cisplatin by targeting ITSN1.

关 键 词：miR-10a-5p ITSN1 宫颈癌 耐药 

分 类 号：R737.33[医药卫生—肿瘤]