LASP1和HIF-1α在肺癌中的表达及临床价值  

Expression and Clinical value of LASP1 and HIF-1α in lung cancer

作　　者：李黎 张文琪[1] 李强[1] 孙瑶[1] LI Li;ZHANG Wen-qi;LI Qiang;SUN Yao(Department of Radiation Oncology,Yidu Central Hospital of Weifang,Weifang 262500,Shangdong,China)

机构地区：[1]潍坊市益都中心医院放疗科,山东潍坊262500

出　　处：《广东医学》2020年第24期2569-2573,共5页Guangdong Medical Journal

摘　　要：目的分析LASP1(LIM and SH3 protein 1)和缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)在肺癌中的表达及其与临床因素之间的相关性。方法应用qRT-PCR、Western blot分别检测肺癌细胞株和正常肺细胞株间LASP1和HIF-1αmRNA和蛋白表达差异,免疫组化分析肺癌患者LASP1和HIF-1α蛋白表达情况。结果 LASP1和HIF-1αmRNA和蛋白在肺细胞株中的表达明显低于在肺癌细胞株中的表达(P<0.05)。肺癌患者中LASP1表达与N分期(P=0.033)密切相关,HIF-1α与LASP1表达与T分期(P=0.027)和N分期(P=0.015)密切相关;LASP1和HIF-1α表达呈正相关(r=0.273,P=0.025)。预后生存分析发现N分期、LASP1和HIF-1α表达为本组肺癌患者预后独立因素。结论肺癌中LASP1和HIF-1α表达升高,LASP1和HIF-1α表达可作为肺癌预后预测指标。Objective To investigate the expression of LASP1(LIM and SH3 protein 1) and HIF-1α in lung cancer and to determine the correlation of LASP1 and HIF-1α expression with clinicopathological characteristics and prognosis.Methods The levels of LASP1 and HIF-1α were detected by real-time quantitative reverse transcription PCR and Western blot. In addition, LASP1 and HIF-1α were also analyzed by immunohistochemistry in formalin-fixed samples. Results The mRNA and protein expression levels of LASP1 and HIF-1α were significantly upregulated in lung cancer cell lines compared with normal lung cell line. There was a significantly positive correlation between LASP1 expression and HIF-1α expression in lung cancer tissues. In addition, N stage were identified as the relative factors of LASP1 expression, while T stage and N stage were identified as the relative factors of HIF-1α expression in lung cancer tissues. There was a significantly positive correlation between LASP1 expression and HIF-1α expression in lung cancer tissues. Multivariate Cox analysis indicated that N stage, LASP1 expression and HIF-1α expression were independentprognostic indicators for lung cancer. Conclusions LASP1 and HIF-1α were overexpressed in NSCLC and may be promising indicators of prognosis for lung cancer patients.

关 键 词：肺癌 LASP1 HIF-1Α 预后 

分 类 号：R734.2[医药卫生—肿瘤] R446.62[医药卫生—临床医学]