木薯中lncRNA-CRR5在低温胁迫下的功能分析  

Identification of lncRNA-CRR5 in Response to Low TemperatureStress in Cassava

作　　者：沈婕 董世满 李淑霞 彭明[1] SHEN Jie;DONG Shiman;LI Shuxia;PENG Ming(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;College of Tropical Crops,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]海南大学热带作物学院,海南海口570228

出　　处：《热带作物学报》2021年第1期40-46,共7页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金青年基金项目(No.31701484);国家自然科学基金国际合作与交流项目(No.31561143012);国家重点研发计划项目(No.2018YFD1000500)。

摘　　要：低温胁迫严重影响木薯的生长、产量以及块根品质。长链非编码RNAs(lncRNAs)作为关键的调节因子,在植物响应非生物胁迫过程中发挥重要的功能。然而,lncRNAs在木薯中的功能和调控机制尚无报道。因此,为了研究木薯lncRNAs在低温胁迫应答中的功能,本研究以木薯主栽品种‘60444’为材料,利用低温处理前后的叶片转录组数据,鉴定到一个受低温胁迫调控的lncRNA(cold-responsive lncRNA5,CRR5)。通过对其序列比对进行同源性分析,RNA二级结构预测和共表达分析鉴定来分析lncRNA-CRR5响应低温胁迫的分子功能。并根据基因的共表达分析推测CRR5的顺式作用和反式作用靶基因。这些结果为更进一步研究CRR5的功能提供了理论依据。Low temperature stress seriously affects the growth and yield of cassava and the quality of roots.Long non-coding RNAs(lncRNAs)function as major regulators,have been shown to play essential roles in plants in response to abiotic stresses.However,the function and regulation mechanism in cassava has not been reported yet.Therefore,in order to investigate the function of lncRNAs in low-temperature response,we identified a cold-responsive lncRNA 5(CRR5)from the transcriptome data using cassava cultivars‘60444’as the material.We analyzed the molecular function of lncRNA-CRR5 in response to low temperature stress by homology analysis,RNA secondary structure prediction and co-expression analysis.The cis-acting target gene and trans-acting target gene of CRR5 were predicted based on the analysis of gene co-expression.The results would provide a new perspective theoretical basis for further exploring the response of CRR5 to low temperature.

关 键 词：木薯 低温胁迫 lncRNA 功能分析 同源性分析 RNA二级结构预测 共表达基因分析 靶基因 

分 类 号：S533[农业科学—作物学]