紫外线照射对两种钛种植体表面形貌体外炎症反应的影响  

Effects of two ultraviolet-treated titanium implant topographies on macrophage inflammatory response in vitro

作　　者：吕武龙[1] 邓炜[2] 雷雳[2] 刘大勇[3] 运新跃[1] 李长义[1] LYU Wu-long;DENG Wei;LEI Li;LIU Da-yong;YUN Xin-yue;LI Chang-yi(Department of General Dentistry,Hospital of Stomatology,Tianjin Medical University,Tianjin 300070,China;Department of Stomatology,The First Affiliated Hospital of Gannan Medical University,Jiangxi Province 341000,China;Department of Endodontics,Hospital of Stomatology,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学口腔医院口腔综合科,天津300070 [2]赣南医学院第一附属医院口腔科,江西341000 [3]天津医科大学口腔医院牙体牙髓科,天津300070

出　　处：《口腔颌面修复学杂志》2021年第1期24-27,32,共5页Chinese Journal of Prosthodontics

基　　金：国家自然科学基金(项目编号:31470920,81870809)天津医科大学科研基金(项目编号:2015KYZM04)。

摘　　要：目的:紫外线照射的抛光试件和TiO2纳米管试件对巨噬细胞增殖和细胞因子分泌的影响。方法:抛光组和纳米管组试件避光保存后,各取一半经紫外线照射获得抛光+紫外线组和纳米管+紫外线组试件。测量各组试件表面接触角;在各组试件上培养RAW264.7巨噬细胞,另设空白对照组。检测培养4、24和72h时巨噬细胞黏附和增殖情况;液相芯片技术检测培养24和72h时细胞上清液中肿瘤坏死因子α(TNF-α)、血管内皮生长因子(VEGF)和巨噬细胞炎性蛋白1α(MIP-1α)的质量浓度。结果:抛光+紫外线组和纳米管+紫外线组试件的接触角(39.6°±3.8°和0.0°±0.0°)均显著小于抛光组和纳米管组。4和24h时五组细胞黏附和增殖结果相似,72h时纳米管+紫外线组细胞增殖显著大于其他四组;培养72h时,抛光+紫外线组和纳米管+紫外线组TNF-α和VEGF质量浓度均分别显著小于抛光组和纳米管组,抛光组MIP-1α质量浓度显著高于其他四组(P<0.05)。结论:紫外线照射能增加两种形貌尤其是TiO2纳米管形貌的亲水性。紫外线照射的TiO2纳米管形貌能促进巨噬细胞增殖。紫外线照射的两种钛种植体表面形貌能减轻炎症反应。Objective:To investigate the effects of ultraviolet(UV)-treated polished titanium(Ti)and TiO2 nanotubular surfaces on macrophage proliferation,secretion of inflammatory cytokines.Methods:The samples of Polished Ti and Nanotubes groups were stored in dark.Half of the samples from each group were treated with UV light for 48 h(Polished Ti+UV group and Nanotubes+UV group),with UV-untreated samples served as control.Contact angle measurements were carried out.RAW 264.7 macrophages were cultured on four kinds of samples and blank group.Cell adhesion and proliferation were conducted using CCK-8 assay after 4,24 and 72 h culture.Levels of cytokine(TNF-α,VEGF and MIP-1α)secreted into the supernatant were measured at 24 and 72 h using magnetic luminex assay.Results:Contact angles in the Polished Ti+UV and Nanotubes+UV groups(39.6°±3.8°and 0.0°±0.0°)were significantly smaller than those in the UV-untreated groups(P<0.05).Cell adhesion at 4 h and proliferation at 24 h were similar in all groups,proliferation in Nanotubes+UV group at 72 h was significantly higher than those in other four groups.Concentrations of TNF-αand VEGF in the Polished Ti+UV and Nanotubes+UV groups at 72 h were significantly lower than those in UV-untreated groups respectively.MIP-1αconcentration in the Polished Ti group at 72 h was significantly higher than those in other four groups(P<0.05).Conclusions:UV treatment can increase hydrophilicity of two titanium surface topographies,especially of Nanotubes+UV group.UV-treated TiO2 nanotubular surface can facilitate macrophage proliferation.Two UV-treated titanium implant surface topographies reduce the inflammatory response in vitro.

关 键 词：紫外线 钛种植体 巨噬细胞 炎症反应 

分 类 号：R783.4[医药卫生—口腔医学]