miR-204过表达对喉鳞癌细胞增殖、周期、凋亡及侵袭的影响及机制  

Effects of miR-204 overexpression on proliferation,cell cycle,apoptosis,and invasion of laryngeal squamous cell carcinoma cells and the mechanism

作　　者：罗德艳[1] 冯俊[1] 彭涛[1] 唐一萍[1] 杨久梅 LUO Deyan;FENG Jun;PENG Tao;TANG Yiping;YANG Jiumei(The Second Clinical Medical College of North Sichuan Medical College·Nanchong Central Hospital,Nanchong 637000,China)

机构地区：[1]川北医学院第二临床医学院·南充市中心医院,四川南充637000

出　　处：《山东医药》2021年第4期36-39,共4页Shandong Medical Journal

基　　金：四川省科技厅重点研发项目(2018FZ0116);四川省教育厅自然科学重点项目(18ZA203);南充市科技局应用技术研究与开发基金项目(16YFZJ0021)。

摘　　要：目的探讨微小RNA-204(miR-204)过表达对喉鳞状细胞癌(鳞癌)细胞增殖、凋亡、侵袭的影响及机制。方法取人喉鳞癌细胞株Hep-2,分为miR-204模拟物组、阴性对照组、空白对照组,分别将miR-204 mimics、NCmimics转染至miR-204模拟物组、阴性对照组,空白对照组未转染。用qRT-PCR法检测各组miR-204相对表达量,分别采用CCK8实验、平板克隆形成实验、流式细胞术、Transwell实验检测miR-204过表达对Hep-2细胞增殖、细胞周期、凋亡及侵袭的影响,采用Western blotting法检测各组高迁移率组蛋白A2(HMGA2)相对表达量。结果转染后,与空白对照组、阴性对照组比较,miR-204模拟物组miR-204相对表达量高,细胞增殖能力、细胞克隆数低,G0/G1期细胞比例高,S期、G2/M期细胞低,细胞凋亡率高,侵袭细胞数低,HMGA2蛋白相对表达量低(P均<0.05)。空白对照组与阴性对照组以上各指标比较差异均无统计学意义(P均>0.05)。结论miR-204过表达能够抑制喉鳞癌Hep-2细胞增殖、侵袭,促进其凋亡,其作用机制可能与调控HMGA2表达有关。Objective To investigate the effects and mechanism of microRNA-204(miR-204)overexpression on proliferation,apoptosis,and invasion of laryngeal squamous cell carcinoma(LSCC)cells.Methods Human laryngeal squamous cell carcinoma cell line Hep-2 was divided into the miR-204 mimics group,negative control group,and blank control group.MiR-204 mimics and NC-mimics were transfected into Hep-2 cells,respectively,and the cells in the blank control group were not treated.The qRT-PCR was used to detect the relative expression of miR-204 in Hep-2 cells.CCK-8 assay,plate clone formation test,flow cytometry,and Transwell invasion test were used to detect the effects of miR-204 overexpression on proliferation,cell cycle,apoptosis,and invasion of Hep-2 cells.Western blotting was used to detect the relative expression level of high-mobility group protein A2(HMGA2)in Hep-2 cells.Results After transfection,compared with the blank control group and negative control group,miR-204 mimics group had higher relative expression of miR-204,lower cell proliferation ability and cell clone number,higher proportion of cells in G0/G1 phase,lower proportion of cells in the S phase and G2/M phase,higher apoptosis rate,lower number of invasive cells,and lower relative expression of HMGA2 protein(all P<0.05).There was no significant difference in the above indexes between the negative control group and the blank control group(all P>0.05).Conclusion Overexpression of miR-204 can inhibit the proliferation and invasion,and promote apoptosis of Hep-2 cells;the mechanism may be related to the regulation of HMGA2 expression.

关 键 词：喉鳞状细胞癌 微小RNA-204 细胞增殖 细胞周期 细胞凋亡 细胞侵袭 高迁移率组蛋白A2 

分 类 号：R739.6[医药卫生—肿瘤]