5-Fu在新一代结肠癌PDX模型中治疗剂量的研究  

Study on the therapeutic dose of 5-fluorouracil in a new generation PDX model of colon cancer

作　　者：刘靖 陈战 李鹏[2] 李迪 涂永久 LIU Jing;CHEN Zhan;LI Peng;LI Di;TU Yong-jiu(Department of General Surgery,Chenggong Hospital Affiliated to Xiamen University,Xiamen Fujian 361000;Department of General Surgery,Xiang′an Hospital Affiliated to Xiamen University,Xiamen Fujian 361102,China)

机构地区：[1]厦门大学附属成功医院普通外科,福建厦门361000 [2]厦门大学翔安附属医院普通外科,福建厦门361102

出　　处：《蚌埠医学院学报》2021年第1期10-13,17,共5页Journal of Bengbu Medical College

摘　　要：目的:建立结直肠癌病人来源的异种移植(PDX)模型,探讨氟尿嘧啶(5-fluorouracil,5-Fu)的药效、毒性与给药剂量之间的关系。方法:将新鲜的结直肠癌手术标本移植至免疫缺陷小鼠皮下,建立PDX模型并稳定传代,检测模型的溯源性;分别给予5、10、20、40、60 mg/kg浓度的5-Fu,测量肿瘤体积、小鼠体质量变化和死亡情况等。结果:成功建立PDX模型,溯源性高达99.96%。5-Fu 5、10、20、40、60 mg/kg剂量组对应的肿瘤抑制率分别为33%、49%、68%、92%、100%。其中,40 mg/kg剂量组小鼠体质量剧减,60 mg/kg剂量组死亡率达100%。结论:5-Fu的体内抑瘤效果及毒性与剂量密切相关,10~20 mg/kg剂量的5-Fu较适合PDX模型的药效评价。Objective:To establish the patient-derived tumor xenografts(PDX)model of colorectal cancer(CRC),and explore the relationship among the anticancer effects,toxicity and dose of 5-fluorouracil(5-Fu).Methods:The fresh human CRC tissues were subcutaneously transplanted into immunodeficient mice to establish a PDX model,and the traceability of the test model was detected.The mice were treated with 5,10,20,40 and 60 mg/kg of 5-Fu,respectively,and the tumor volume,body mass and mortality were measured.Results:The PDX model was successfully established,and the traceability was 99.96%.The tumor inhibition rates of 5,10,20,40 and 60 mg/kg of 5-Fu were 33%,49%,68%,92%and 100%,respectively.Among them,the body weight of mice significantly lost in 40 mg/kg dose group,and the mortality of 60 mg/kg dose group was 100%.Conclusions:The efficacy and toxicity of 5-Fu are closely related to the doses,and the 10-20 mg/kg of 5-Fu is suitable for the efficacy evaluation of PDX model.

关 键 词：结直肠肿瘤 氟尿嘧啶 病人来源肿瘤异种移植 

分 类 号：R735.3[医药卫生—肿瘤]