辐射干预后C6胶质瘤干细胞wnt5a阳性表达的实验研究  

Experimental Study on the Positive Expression of wnt5a in C6 Glioma Stem Cells after Radiation Intervention

作　　者：丁银秀 刘平[2] 程莹莹 刘印明 冯利强[2] DING Yin-xiu;LIU ping;CHENG Ying-ying;LIU Yin-ming;FENG Li-qiang(Ningxia Hui Autonomous Region Institute of Medical Sciences,Ningxia Yinchuan 750000;School of Basic Medicine of Ningxia Medical University,Ningxia Yinchuan 750006)

机构地区：[1]宁夏回族自治区医学科学研究所,宁夏银川750000 [2]宁夏医科大学基础医学院,宁夏银川750006

出　　处：《深圳中西医结合杂志》2020年第24期3-5,F0003,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金地区项目资助课题(31760279,81360062);宁夏自然科学基金项目资助课题(2018AAC03207)。

摘　　要：目的:探讨C6胶质瘤干细胞(C6 GSCs)的辐射干预后wnt5a分子阳性表达的情况。方法:BrdU掺入实验明确细胞增殖情况;共聚焦显微观察wnt5a的细胞定位;蛋白电泳测定wnt5a蛋白表达水平。结果:条件培养基的作用下,C6 GSCs数量达到98 %以上;辐射干预后,0.3 Gy组促进了C6 GSCs的增殖和分化能力;3.0 Gy组细胞的增殖和分化能力下降;wnt5a在细胞核、胞浆和突起上均有表达,0.3 Gy组与对照组比较,差异具有统计学意义(P<0.05);蛋白电泳灰度统计分析与上述结果一致。结论:一定范围内的低剂量电离辐射刺激对C6 GSCs的增殖、分化具有促进作用,且wnt5a分子在C6 GSCs上呈阳性表达,提示wnt分子可能参与介导C6 GSCs电离辐射的刺激效应。Objective To investigate the positive expression of wnt5a molecules after radiation intervention of C6 glioma stem cells (C6 GSCs).Methods BrdU incorporation experiment to clarify the cell proliferation;confocal microscopy to observe the cell location of wnt5a;protein electrophoresis to determine the level of wnt5a protein expression.Results Under the effect of conditioned medium,the number of C6 GSCs reached more than 98%;after radiation intervention,the 0.3 Gy group promoted the proliferation and differentiation of C6 GSCs;the 3.0 Gy group decreased the proliferation and differentiation;wnt5a was found in the nucleus,cytoplasm and There are expressions on the protrusions,and the difference between the 0.3 Gy group and the Con group is statistically significant (P < 0.05);the gray-scale statistical analysis of protein electrophoresis is consistent with the above results.Conclusion A certain range of low-dose ionizing radiation stimulation can promote the proliferation and differentiation of C6 GSCs,and wnt5a molecules are positively expressed on C6 GSCs,suggesting that wnt molecules may be involved in mediating the stimulation effects of C6 GSCs ionizing radiation.

关 键 词：C6胶质瘤干细胞 BrdU掺入实验 低剂量电离辐射技术 

分 类 号：R739[医药卫生—肿瘤]