基因芯片筛选新疆哈萨克族食管癌与癌旁正常组织间差异表达miRNA及其功能验证  

Screening of differentially expressed miRNA between esophageal carcinoma and para-carcinoma normal tissues of Kazak ethnic group of Xinjiang and its functional verification

作　　者：刘涛[1,2] 王淑霞 卢晓梅[1,2] 王育珊 苏银霞[1,2] 姚华 LIU Tao;WANG Shuxia;LU Xiaomei;WANG Yushan;SU Yinxia;YAO Hua(The First Affiliated Hospital of Xinjiang Medical University,Urumqi.830054,China;Health Management Institute.Xinjiang Medical University,Urumqi,830054,China)

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830054 [2]新疆医科大学健康管理院,乌鲁木齐830054

出　　处：《新疆医学》2020年第10期1005-1009,共5页Xinjiang Medical Journal

基　　金：国家自然科学基金(项目编号:81860510);省部共建国家重点实验室开放课题(项目编号:SKL-HIDCA-2017-Y9)。

摘　　要：目的基因芯片筛选新疆哈萨克族食管癌组织和癌旁正常组织差异表达miRNA及其功能验证,为进一步研究miRNAs在食管癌发生发展中的作用机制提供理论依据。方法收集6对新疆哈萨克族食管癌患者癌组织及癌旁正常组织,miRNA微阵列芯片分析食管癌组织及癌旁正常组织的差异miRNA,实时荧光定量PCR验证miR-155在食管癌组织及癌旁组织中的表达,并在细胞水平上采用四甲基偶氮唑盐微量酶反应比色法、细胞划痕、和细胞周期实验验证其与食管癌恶性表型的相关性。结果与癌旁正常组织相比,共筛选出差异miRNA 69个,其中包括上调miRNA 56个,下调miRNA13个。并对筛选出的上调基因miR-155进行实时荧光定量PCR验证和细胞功能验证,miR-155在癌组织中表达明显高于癌旁组织,与芯片结果一致;并且在细胞水平上促进了食管癌细胞增殖、迁移,阻值食管癌细胞于S期。结论基因芯片筛选并验证了miR-155参与了食管癌的恶性表型。Objective To screen differentially expressed miRNA and its functional verification in esophageal cancer tissues and adjacent normal tissues of Kazak ethnic group of Xinjiang and provide theoretical basis for further research on the mechanism of miRNAs in the development of esophageal cancer.Methods Six pairs of cancer tissues and adjacent normal tissues of Kazakh patients with esophageal cancer in Xinjiang were collected.MiR NA microarray was used to analyze the differential miRNAs between esophageal cancer tissues and normal adjacent tissues.Real time quantitative PCR was used to verify the expression of miR-155 in esophageal cancer tissues and adjacent normal tissues,At the cellular level,the correlation between the expression and the malignant phenotype of esophageal cancer was verified by MTT,cell scratch test and cell cycle test.Results A total of 69 miRNAs were screened out,including 56 up-regulated miRNAs and 3 down regulated miRNAs.The results showed that the expression of miR-155 in cancer tissues was significantly higher than that of adjacent tissues,which was consistent with the microarray results.Moreover,miR-155 promoted the proliferation and migration of esophageal cancer cells at the cellular level,and prevented the esophageal cancer cells in S phase.Conclusions miR-155 is involved in the malignant phenotype of esophageal cancer.

关 键 词：基因芯片 MIRNA 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]