成纤维细胞生长因子5对胰腺癌细胞生长和肝转移能力的影响  

Effects of fibroblast growth factor 5 on growth and hematogenous metastasis of human pancreatic cancer cells

作　　者：武颖超[1] 文龙 朱静[1] 汪欣[1] Wu Yingchao;Wen Long;Zhu Jing;Wang Xin(Department of General Surgery,Peking University First Hospital,Beijing 100034,China;Department of Neurosurgery,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院普通外科,100034 [2]北京大学第一医院神经外科,100034

出　　处：《中华实验外科杂志》2021年第1期17-19,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察成纤维细胞生长因子5(FGF5)对人胰腺癌细胞生长和肝转移能力的影响。方法利用高表达FGF5的人胰腺癌PANC-1细胞系(北京大学第一医院外科大肠癌实验室提供),通过短发卡RNA(shRNA)稳定转染技术,构建FGF5低表达细胞系PANC-1-FGF5-,并以未转染质粒和转染对照粒的PANC-1细胞作为对照。通过皮下注射各组细胞建立裸鼠皮下种植瘤模型;通过脾脏内注射各组细胞建立肝转移模型,6周后切取皮下种植瘤体并称重,切取肝转移标本,计数肝转移灶数目。单因素方差分析比较各组裸鼠皮下种植瘤质量和肝转移灶个数的差异,Spearman等级相关性分析各组裸鼠皮下种植瘤质量和肝转移灶个数与各组细胞FGF5表达水平之间的相关性。结果蛋白质印迹法(Western blot)法证实FGF5低表达及对照质粒细胞构建成功;PANC-1-FGF5-组皮下种植瘤质量[(0.42±0.19)g]明显低于Pan-1组[(1.35±0.45)g]和PANC-1-Vector组[(1.32±0.35)g],差异有统计学意义(F=18.506,P<0.01);PANC-1-FGF5-组肝转移灶数目[(7.50±4.11)个]明显少于PANC-1组[(26.25±9.78)个]和PANC-1-Vector组[(26.25±9.78)个],差异有统计学意义(F=12.971,P<0.01);种植瘤质量和肝转移灶数与FGF5的表达量成正相关(r=0.818、0.806,P<0.01)。结论FGF5在裸鼠体内能够促进胰腺癌细胞的生长和肝转移能力。Objective To explore the effects of the fibroblast growth factor 5(FGF5)on the growth and the hematogenous metastasis of the human pancreatic cancer cells(PANC-1 cells).Methods PANC-1 cells were stably transfected with FGF5 short hairpin RNA(shRNA)and contrast vector,respectively.Transfected cells were selected with G418.Cells from each group(PANC-1,PANC-1-Vector and PANC-1-FGF5-cells)were inoculated subcutaneously into 8 nude mice(bagg′s albino,BALB/c nu/nu)respectively.The subcutaneous tumors were harvested and weighed.Cells from each group were injected into spleens of 8 nude mice by operations.Livers were harvested 6 weeks after nude mice liver metastasis model was constructed.Then the liver metastatic amounts were countered macroscopically.The quality of subcutaneous tumor and the number of liver metastases in each group were compared by one way analysis of variance(ANOVA),and the correlation between the quality of subcutaneous tumor and the number of liver metastases and the expression level of FGF5 was analyzed by Spearman correlation analysis.Results Western blotting showed that FGF5 shRNA and contrast vector cell lines were constructed successfully.The quality of the subcutaneous tumors of PANC-1-FGF5-cells[(0.42±0.19)g]was less than PANC-1 cells[(1.35±0.45)g]and PANC-1-Vector cells[(1.32±0.35)g],and the difference was significant(F=18.506,P<0.01).The liver metastatic amounts of PANC-1-FGF5-cells(7.50±4.11)were less than PANC-1 cells(26.25±9.78)and PANC-1-Vector cells(26.25±9.78),and the difference was significant(F=12.971,P<0.01).The expression of FGF5 was highly positively correlated with the quality of the tumors and liver metastatic amounts(r=0.818,0.806,P<0.01).Conclusion FGF5 could promote the growth and the liver metastasis of PANC-1 cells in vivo in nude mice.

关 键 词：胰腺肿瘤 成纤维细胞生长因子 转移 生长 转染 

分 类 号：R735.9[医药卫生—肿瘤]