Twist1、SIRT1、FGF2、TGF-β3基因在胎盘、脐带和乳牙三种间充质干细胞的表达及增殖调控作用研究  

Expression of Twist1,SIRT1,FGF2 and TGF-β3 genes and its regulatory effect on the proliferation of placenta,umbilical cord and dental pulp mesenchymals stem cells

作　　者：谭遥 邓银 彭柯又 孙正周 黄健秋 辜勋童 张富胜 彭汉卿 张学超 张蓉[3] Tan Yao;Deng Yin;Peng Keyou;Sun Zhengzhou;Huang Jianqiu;Gu Xuntong;Zhang Fusheng;Peng Hanqing;Zhang Xuechao;Zhang Rong(Chengdu Zhongchuang Qingke Medical Diagnose Test Center,Chengdu,Sichuan 610041,China;Chengdu Qingke Biotechnology Co.Ltd.,Chengdu,Sichuan 610094,China;Department of Biochemistry and Molecular Biology,College of Basic and Forensic Medicine,Sichuan University,Chengdu,Sichuan 610041,China)

机构地区：[1]成都中创清科医学检验所有限公司,610041 [2]成都清科生物科技有限公司,610041 [3]四川大学基础医学与法医学院生物化学与分子生物学教研室,成都610041

出　　处：《中华医学遗传学杂志》2021年第2期117-122,共6页Chinese Journal of Medical Genetics

摘　　要：目的比较细胞增殖相关基因Twist1、SIRT1、FGF2、TGF-β3在胎盘、脐带和乳牙3种间充质干细胞中的表达差异,分析其对3种间充质干细胞的增殖调控作用。方法通过显微镜对不同传代次数的3种间充质干细胞的形态结构进行比较;经过体外传代培养,利用荧光定量PCR测定细胞增殖相关基因Twist1、SIRT1、FGF2、TGF-β3的表达水平;采用MTT法检测并比较分析3种间充质干细胞的增殖及促增殖能力。结果Twist1、TGF-β3基因在胎盘间充质干细胞中表达水平最高,FGF2基因的表达水平最低,SIRT1基因在脐带间充质干细胞中的表达水平较高。随着传代培养进行,4种基因表达水平在不同代次的间充质干细胞中的表达水平各不相同。Twist1、SIRT1、TGF-β3表达上调时3种间充质干细胞增殖能力增强,FGF2表达上调时3种间充质干细胞增殖能力减弱。结论Twist1、SIRT1、FGF2、TGF-β3在3种间充质干细胞中的表达水平存在一定差异;Twist1、SIRT1、TGF-β3对3种间充质干细胞的增殖表现为正调控作用;而FGF2基因则相反表现为负调控作用。Objective To compare the mRNA level of cell proliferation-related genes Twist1,SIRT1,FGF2 and TGF-β3 in placenta mesenchymals stem cells(PA-MSCs),umbilical cord mensenchymals(UC-MSCs)and dental pulp mesenchymals stem cells(DP-MSCs).Methods The morphology of various passages of PA-MSCs,UC-MSCs and DP-MSCs were observed by microscopy.Proliferation and promoting ability of the three cell lines were detected with the MTT method.Real-time PCR(RT-PCR)was used to determine the mRNA levels of Twist1,SIRT1,FGF2,TGF-β3.Results The morphology of UC-MSCs and DP-MSCs was different from that of PA-MSCs.Proliferation ability and promoting ability of the PA-MSCs was superior to that of UC-MSCs and DP-MSCs.In PA-MSCs,expression level of Twist1 and TGF-β3 was the highest and FGF2 was the lowest.SIRT1 was highly expressed in UC-MSCs.With the cell subcultured,different expression levels of Twist1,SIRT1,FGF2,TGF-β3 was observed in PA-MSCs,UC-MSCs and DP-MSCs.Conclusion Up-regulated expression of the Twist1,SIRT1 and TGF-β3 genes can promote proliferation of PA-MSCs,UC-MSCs and DP-MSCs,whilst TGF-β3 may inhibit these.The regulatory effect of Twist1,SIRT1,FGF2 and TGF-β3 genes on PA-MSCs,UC-MSCs and DP-MSCs are different.

关 键 词：间充质干细胞 传代培养 细胞增殖 基因表达 

分 类 号：R73[医药卫生—肿瘤]