蛋白质中氨基酸外消旋化修饰的非特定位点分析方法研究进展  

Advances in Research on Non-site-specific Determination of Amino Acids Racemization Modification in Proteins

作　　者：王佳凤[1,2] 王生伟 李博[1,2] 狄斌[1,2] WANG Jiafeng;WANG Shengwei;LI Bo;DI Bin(Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009,China;Key Laboratory of Protein Chemistry and Structural Biology,China Pharmaceutical University,Nanjing 210009,China;Emergency Department,Nanjing Chest Hospital,Nanjing 210029,China)

机构地区：[1]中国药科大学药物分析系,江苏南京210009 [2]中国药科大学蛋白质化学与结构生物学重点实验室,江苏南京210009 [3]南京市胸科医院急诊科,江苏南京210029

出　　处：《药学进展》2020年第11期854-860,共7页Progress in Pharmaceutical Sciences

摘　　要：生物体内蛋白质的D-氨基酸残基主要通过外消旋化产生,而外消旋化修饰也被证明与白内障等疾病的发生和发展具有相关性。常见的蛋白质外消旋化修饰分析方法包括蛋白质整体外消旋化修饰分析、特定位点外消旋化修饰分析和非特定位点外消旋化修饰分析等几种类型。非特定位点外消旋化修饰分析对于全面探究体内外蛋白质中外消旋化修饰规律、评价外消旋化修饰对蛋白质功能的影响,以及阐明重大疾病与蛋白质外消旋化的关系均具有重要意义。综述了近年来非特定位点外消旋化修饰分析方法的研究进展,将其与蛋白质整体外消旋化修饰分析方法、特定位点外消旋化修饰分析方法进行对比,并对非特定位点外消旋化分析方法进行了展望。D-amino acid residues of protein are mainly produced by amino acid racemization(AAR),which has been proved to be related to the occurrence and development of cataracts and other diseases.Common analytical methods of protein AAR modification include whole protein AAR analysis,site-specific AAR analysis and non-site-specific AAR analysis.Non-site-specific determination of AAR modification is very important for the comprehensive exploration of in vivo and in vitro protein AAR modification.It is also significant to evaluate the effects of AAR modification on protein function and clarify the relationship between major diseases and protein AAR.In this paper,the research progresses of analytical methods of non-site-specific AAR modification in recent years are reviewed,in comparison with whole protein AAR and site-specific AAR modification analysis.The prospect of the non-site-specific AAR analysis is also discussed.

关 键 词：蛋白质 氨基酸 非特定位点 外消旋化 

分 类 号：TQ937[化学工程]