颅脑损伤后弥漫性轴索损伤的CT表现与患者神经元水通道蛋白表达的相关性  

The correlation between CT findings of diffuse axonal injury and the expression of neuronal aquaporin in patients with craniocerebral injury Seventh

作　　者：阮娇妮 宋黎涛[1] RUAN Jiao-ni;SONG Li-tao(People's Hospital of Shanghai University of Tradi-tional Chinese Medicine,Shanghai 200137,China)

机构地区：[1]上海中医药大学附属第七人民医院医学影像科,上海200137

出　　处：《中国实验诊断学》2021年第1期93-97,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨颅脑损伤后弥漫性轴索损伤的CT表现与患者神经元水通道蛋白表达的相关性。方法选取经CT检查和临床诊断为弥漫性轴索损伤的150例患者及50例健康体检者为研究对象,根据颅脑CT和GCS量表评分将患者分为DAI轻型、中型及重型三组。观察并记录患者一般情况,比较各组患者脑组织病理形态、颅脑水肿情况和CT影像结果。通过RT-PCR和Western blot法检测了患者血清及脑组织中AQP-4水平的变化,探讨DAI的CT表现与患者神经元水通道蛋白表达的相关性。结果 DAI病理形态、颅脑水肿及CT影像结果表明,DAI各组脑组织均有一定程度的损伤,且随着损伤程度的增加,水肿程度及轴索损伤数量急剧增加,差异显著(P<0.05),CT可作为DAI快速高效诊断的有效依据。RT-PCR、Western blot检测及Spearman相关性分析表明,各DAI患者组血清及脑组织中AQP-4水平显著提高;随着弥漫性轴索损伤程度的增加,AQP-4表达水平进一步提高,差异显著(P<0.05);各组患者CT表现与AQP-4蛋白表达水平呈明显正相关。结论弥漫性轴索损伤的CT影像表现与水通道蛋白表达水平有明显相关性,AQP-4可作为判断DAI病情及预后的一个重要分子指标,为临床诊断及治疗DAI提供了一种新的无创检测手段,具有较高的临床应用价值。Objective Explore the correlation between CT findings of diffuse axonal injury and the expression of neuronal aquaporin in patients with craniocerebral injury.Methods 150 patients with diffuse axonal injury diagnosed by CT and 50 healthy physical examinators were selected as the study objects.According to the craniocerebral CT and GCS scale scores,the patients were divided into DAI light,medium and heavy groups.The general conditions of the patients were observed and recorded,and the brain pathological morphology,craniocerebral edema and CT imaging results of the patients in each group were compared.Changes in serum and brain aqp-4 levels were detected by RT-PCR and Western blot,and the correlation between CT manifestations of DAI and the expression of neuronal aquaporin was investigated.Results The results of DAI’s pathological morphology,cerebral edema and CT imaging showed that the brain tissue of each group of DAI had a certain degree of injury,and with the increase of the injury degree,the degree of edema and the number of axonal injury increased sharply,with significant differences(P<0.05).Therefore,CT could be used as an effective basis for the rapid and efficient diagnosis of DAI.RT-PCR,Western blot and Spearman correlation analysis showed that the levels of aqp-4 in the serum and brain tissue of DAI patients were significantly increased.With the increase of the degree of diffuse axonal injury,the expression level of aqp-4 was further increased,and the difference was significant(P<0.05).The CT manifestations of patients in each group were positively correlated with the expression level of aqp-4 protein.Conclusion AQP-4 can be used as an important molecular index to judge the condition and prognosis of Dai.It provides a new non-invasive detection method for clinical diagnosis and treatment of Dai,and has high clinical application value.

关 键 词：弥漫性轴索损伤 CT 水通道蛋白 

分 类 号：R575.1[医药卫生—消化系统]