MicroRNA-21-5p和MicroRNA-18b-5p在大肠癌中的表达及意义  

Expression and significance of MicroRNA-21-5p and MicroRNA-18b-5p in colorectal cancer

作　　者：刘日旭[1] 王乙[1] 曹岩[1] 刘明开[1] LIU Ri-xu;WANG Yi;CAO Yan(Department of Clinical Laboratory,Xinhua Hospital Affilicated to Dalian Universily,Dalian 116021,China)

机构地区：[1]大连大学附属新华医院检验科,辽宁大连116021

出　　处：《中国实验诊断学》2021年第1期103-105,共3页Chinese Journal of Laboratory Diagnosis

基　　金：大连市医学科学研究计划项目(1811124)。

摘　　要：目的研究MicroRNA-21-5p和MicroRNA-18b-5p在大肠癌中的表达和临床意义。方法选取大肠癌组织和癌旁正常组织50例,应用qPCR法,检测MicroRNA-21-5p和MicroRNA-18b-5p表达水平;应用免疫组化法,检测PI3K表达水平。结果 MicroRNA-21-5p、MicroRNA-18b-5p和PI3K在大肠癌组织中的的表达量显著高于癌旁正常组织(P<0.05),且MicroRNA-21-5p、MicroRNA-18b-5p均和PI3K具有明显正相关性(P<0.05)。结论 MicroRNA-21-5p和MicroRNA-18b-5p通过PI3K/AKT信号通路在大肠癌的发生、发展过程中起重要作用。Objective To investigate the expression and clinical significance of MicroRNA-21-5 p and MicroRNA-18 b-5 p in the progression of colorectal cancer.Methods In 50 cases of colorectal cancer and normal colorectal tissues,the expression level of microRNA-21-5 p and MicroRNA-18 b-5 p in colorectal cancer was detected by qPCR,and the expression level of PI3 Kin colorectal cancer was detected by immunohistochemistry.Results The expression levels of MicroRNA-21-5 p,MicroRNA-18 b-5 p and PI3 Kin colorectal carcinoma were obviously higher than that of in the normal colorectal tissue,with statistical significance(P<0.05);Microrna-21-5 p and Microrna-18 b-5 p were positively correlated with PI3 K(P<0.05).Conclusion Microrna-21-5 p and Microrna-18 b-5 p play an important role in the occurrence and development of colorectal cancer through PI3 K/AKT signaling pathway.

关 键 词：大肠癌 MicroRNA-21-5p MicroRNA-18b-5p 

分 类 号：R34[医药卫生—基础医学] R735[医药卫生—肿瘤]