基于线粒体Cyt b基因序列的棘头梅童鱼种群遗传结构  

Population genetic structure analysis of big head croaker(Collichthyslucidus)based on mitochondrial cytochrome b gene sequences

作　　者：张帅[1,2] 李敏 闫帅[1] 孔啸兰 朱江峰 徐姗楠[1] 陈作志[1,3] ZHANG Shuai;LI Min;YAN Shuai;KONG Xiaolan;ZHU Jiangfeng;XU Shannan;CHEN Zuozhi(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;Key Laboratory of Open-Sea Fishery Development,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Fishery Ecology Environment,Guangzhou 510300,China;College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院南海水产研究所,农业农村部外海渔业开发重点实验室,广东省渔业生态环境重点实验室,广东广州510300 [2]上海海洋大学海洋科学学院,上海201306 [3]南方海洋科学与工程广东省实验室(广州),广东广州511458

出　　处：《中国水产科学》2021年第1期90-99,共10页Journal of Fishery Sciences of China

基　　金：国家重点研发计划项目(2018YFD0900902);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0605);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金项目(2019TS26).

摘　　要：选取线粒体细胞色素b(cytochrome b,Cyt b)基因序列作为分子标记,分析了中国沿海棘头梅童鱼(Collichthys lucidus)的种群遗传结构。2019年9―11月在中国沿海9个采样点共采集棘头梅童鱼342尾,共检测到174个单倍型。通过构建单倍型网络连接图和单倍型进化树发现,棘头梅童鱼可分为北方支系和南方支系。分子方差分析(AMOVA)结果显示,棘头梅童鱼的南北群体存在显著的遗传分化。核苷酸错配分布分析和中性检验结果显示,棘头梅童鱼在更新世经历过种群快速扩张,且估算北方群体和南方群体的种群扩张时间分别在0.799万~1.999万和2.631万~6.577万年前。根据净遗传距离推算出北方群体和南方群体的分化时间在34.5万~86.25万年前。综上得出,中国沿海棘头梅童鱼可分为南北两个种群,在渔业资源开发和管理上需要区别对待。With the collapse of inshore fishery resources,small economic fishes have become the major fish resource in the coastal waters of China.As one of the most important fish resources,big head croaker(Collichthys lucidus)has a wide distribution and high production in Chinese estuaries and is included on the List of China State Key Protected Commercial Sources of Aquatic Animals and Plants.However,the population structure of C.lucidus is still unclear,and knowledge of this is essential for decision-makers to sustainably develop the fishery industry.In the present study,the population genetic structure of C.lucidus in the coastal waters of China was examined based on mitochondrial cytochrome b gene sequences.A total of 342 individuals were sampled from nine localities from September to November 2019,and 174 haplotypes were subsequently identified.The median-joining network and neighbor-joining tree of haplotypes show two significantly divergent clades,Clade N and Clade S,which correspond to the geographical North group and South group,respectively.Analyses of molecular variance(AMOVA)also revealed significant genetic differentiation between the North and South groups(P<0.01).The genetic diversity results indicated that the haplotype diversity of the South group was higher than that of the North group(0.9418 vs.0.8758);however,the results of nucleotide diversity showed the opposite outcome(0.0036 vs.0.0041).Analyses of pairwise FST and Nm revealed significant genetic differentiation between the North and South group populations(P<0.05),with little gene exchange(Nm=0.06).The results of mismatch distribution and neutrality tests show that population expansion occurred for big head croaker along the coast of China in the Pleistocene era,and the expansion times of the North and South groups were estimated to be 7990-19900 and 26310-65770 years ago,respectively.The divergence time of big head croaker between the North and South groups was estimated at 345000-862500 years ago based on genetic distances.Historical events,biolo

关 键 词：棘头梅童鱼 种群遗传结构 细胞色素B基因 渔业管理 

分 类 号：S931[农业科学—渔业资源]