miR-378-3p靶向调控EZH2表达对血管瘤内皮细胞侵袭和迁移能力的影响  

Effects of miR-378-3p targeting EZH2 expression on invasion and migration of hemangioma endothelial cells

作　　者：史帅涛[1] 王国权[1] 李晓健 韩文豪 翟水亭[1] SHI Shuaitao;WANG Guoquan;LI Xiaojian;HAN Wenhao;ZHAI Shuiting(Department of Vascular Surgery,Central China Fuwai Hospital,Department of Vascular Surgery,Henan Province People’s Hospital,Department of Vascular Surgery,Central China Fuwai Hospital,Zhengzhou University,Zhengzhou 451464)

机构地区：[1]华中阜外医院血管外科,河南省人民医院血管外科,郑州大学华中阜外医院血管外科,郑州451464

出　　处：《郑州大学学报（医学版）》2021年第1期97-101,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关计划项目(2018020440)。

摘　　要：目的:研究miR-378-3p靶向调控果蝇zeste基因增强子同源物2(EZH2)表达影响血管瘤内皮细胞侵袭和迁移能力的机制。方法:分离培养人血管瘤内皮细胞,分成空白对照组、miR-NC组(转染模拟物阴性对照)、miR-378-3p组(转染miR-378-3p模拟物)3组,或miR-378-3p+pcDNA3.1组(共转染pcDNA3.1、miR-378-3p模拟物)、miR-378-3p+pcDNA3.1-EZH2组(共转染pcDNA3.1-EZH2、miR-378-3p模拟物)2组,MTT法测定增殖活性,克隆形成实验检测克隆形成数,Transwell小室检测侵袭细胞数和迁移细胞数。生物信息学数据库预测miR-378-3p与EZH2有互补结合位点,双荧光素酶报告系统鉴定靶向关系。结果:miR-378-3p组与空白对照组、miR-NC组相比,细胞增殖活性、克隆形成数、侵袭细胞数和迁移细胞数降低(P<0.05)。miR-378-3p+pcDNA3.1-EZH2组与miR-378-3p+pcDNA3.1组比较,细胞增殖活性、克隆形成数、侵袭细胞数和迁移细胞数升高(P<0.05)。双荧光素酶报告系统鉴定结果证实miR-378-3p和EZH2存在靶向关系。结论:miR-378-3p靶向下调EZH2表达可抑制血管瘤内皮细胞的增殖、侵袭和迁移能力。Aim:To study the mechanism of miR-378-3p targeting regulation of enhancer of zeste homolog 2(EZH2)expression and affecting the invasion and migration of hemangioma endothelial cells.Methods:The endothelial cells of hemangioma were isolated,cultured and divided into black control group,miR-NC group(transfected mimic negative control),miR-378-3p group(transfected miR-378-3p mimic),or divided into miR-378-3p+pcDNA3.1 group(co-transfected with pcDNA3.1,miR-378-3p mimic),miR-378-3p+pcDNA3.1-EZH2 group(co-transfected with pcDNA3.1-EZH2,miR-378-3p mimic).Proliferation activity was detected by MTT method.The number of colonies formed was detected by clone formation experiment.The number of invaded cells and migrated cells were detected by the Transwell chamber.The bioinformatics database was uesed to predict whether miR-378-3p and EZH2 had complementary binding sites or not,and the double luciferase reporter system was used to identify the targeting relationship.Results:Compared with the black control and the miR-NC group,the miR-378-3p group had lower cell proliferation activity,the number of clones,the number of invaded cells and the number of migrating cells(P<0.05).Compared with the miR-378-3p+pcDNA3.1 group,the cell proliferation activity,the number of clones,the number of invasive cells and the number of migrating cells increased in the miR-378-3p+pcDNA3.1-EZH2 group(P<0.05).The double luciferase reporter system showed that miR-378-3p and EZH2 were mutually targeted.Conclusion:miR-378-3p targeted down-regulating of EZH2 expression could inhibit the proliferation,invasion and migration of hemangioma endothelial cells.

关 键 词：血管瘤内皮细胞 miR-378-3p zeste基因增强子同源物2 侵袭 迁移 

分 类 号：R732.2[医药卫生—肿瘤]