臭氧抑制溶血磷脂酸引起的神经病理性疼痛和背根神经髓鞘相关糖蛋白及TNF-α上调  

Ozone inhibits the neuropathic pain,and upregulation of myline-associated glycoprotein and TNF-αin dorsal root ganglia induced by lysophosphatidic acid

作　　者：陈珊雅 彭良玉[2] 王喜连[1] 周忠群[1] 唐杰[1] 谢斌[1] 陈素昌[1] CHEN Shan-Ya;PENG Liang-Yu;WANG Xi-Lian;ZHOU Zhong-Qun;TANG Jie;XIE Bin;CHENSu-Chang(Department of Pain Medical,First Affiliated Hospital of University of South China,Hengyang 421001,China;Department of Anesthesiology,First Affiliated Hospital of University of South China,Hengyang 421001,China)

机构地区：[1]南华大学附属第一医院疼痛科,衡阳421001 [2]南华大学附属第一医院麻醉科,衡阳421001

出　　处：《中国疼痛医学杂志》2021年第1期41-46,共6页Chinese Journal of Pain Medicine

基　　金：国家青年自然科学基金(81300971);湖南省教育厅项目(18C0430)。

摘　　要：目的:探讨单次椎间孔注射臭氧在溶血磷脂酸(lysophosphatidic acid,LPA)脱髓鞘神经病理性疼痛模型中对背根神经髓鞘相关糖蛋白(myeline-associated glycoprotein,MAG)及肿瘤坏死因子α(tumor necrosis factorα,TNF-α)表达的影响。方法:健康成年雄性小鼠鞘内注射1 nmol LPA进行神经病理性疼痛造模,造模后24 h将小鼠随机分为:人造脑脊液[LPA+aCSF(artificial cerebralspinal fluid)]组和臭氧(LPA+O3)组。同节段椎间孔分别单次注射aCSF或臭氧。在注射LPA前(t0)、注射臭氧或aCSF前(t1)、注射臭氧或aCSF后24 h(t2)、3天(t3)、7天(t4)、14天(t5)时进行疼痛行为学检测(每组8只,共48只),并用蛋白质免疫印迹法在不同时间点(t0-t4)检测背根神经髓鞘蛋白MAG及TNF-α的表达变化(每组3只,共30只)。结果:①与人造脑脊液组对比,臭氧注射后24 h机械缩足反射阈值和热缩足反射潜伏期显著上升,维持到第7天;②与人工脑脊液组对比,臭氧注射后24 h TNF-α表达显著下降,3天时最低,并维持到第7天;③与人工脑脊液组对比,臭氧组注射后24 h和3天MAG的蛋白定量表达稳定,无进一步降解,仅在第7天时稍下降。结论:在溶血磷脂酸脱髓鞘神经病理性疼痛中单次椎间孔注射臭氧通过抑制炎症因子释放以及维持MAG稳定性可以明显缓解神经病理性疼痛。Objective:To investigate the effect of a single intravertebral injection of ozone on the expression of myeline-associated glycoprotein(MAG)and tumor necrosis factorα(TNF-α)in the dorsal root nerve in the demyelinating neuropathy pain model by lysophosphatidic acid(LPA).Methods:Healthy adult male mice were injected with 1 nmol LPA to induce neuropathic pain.Twenty-four hours after modeling,the mice were randomly divided into artificial cerebralspinal fluid(aCSF)group(LPA+aCSF)and ozone group(LPA+O3).With the segmental intervertebral foramen injection of aCSF or ozone,pain behaviors were detected before the injection of LPA(t0),before the ozone injection or aCSF injection(t1),24 hours(t2),3 days(t3),7 days(t4)and 14 days(t5)after the injection of ozone or aCSF(8 mice in each group,48 mice in total).The expression of MAG and TNF-αin the dorsal nerve at different time points(t0-t4)were detected by Western blot(3 mice in each group,30 in total).Results:①Compared with the LPA+aCSF group,the mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were increased significantly 24 hours after ozone injection(t2),and maintained to the 7th day(t4)in LPA+O3 group;②Compared with the LPA+aCSF group,the expression of TNF-αwas significantly decreased 24 hours after ozone injection(t2),being lowest at the 3rd day(t3),and maintained until the 7th day(t4)in LPA+O3 group;③Compared with the LPA+aCSF group,the expression of MAG in the ozone group was stable at 24 h and 3 d after injection(t2 and t3),without further degradation and only slightly decreased on the 7th day(t4)in LPA+O3 group.Conclusion:In the demyelinating neuropathic pain induced by lysophosphatidic acid,the single injection of ozone into the intervertebral foramen can significantly relieve the neuropathic pain by inhibiting the release of inflammatory factor and maintaining the stability of MAG.

关 键 词：溶血磷脂酸 背根神经 髓鞘相关糖蛋白 肿瘤坏死因子Α 臭氧 

分 类 号：R741.041[医药卫生—神经病学与精神病学]