小鼠淋巴细胞内多肽的分离鉴定  

Isolation and Identification of Peptides in Mouse Lymphocytes

作　　者：郭婷婷 栾媛媛 范梦珠 王娟 肖珊珊 陈承余 张少辉[1,2] GUO Tingting;LUAN Yuanyuan;FAN Mengzhu;WANG Juan;XIAO Shanshan;CHEN Chengyu;ZHANG Shaohui(Shanghai Jiao Tong University School of Agriculture and Biology,Shanghai 200240,China;Zhejiang Go Peptides Life Science and Healthcare Co.Ltd.,Wenzhou 325800,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]浙江辉肽生命健康科技有限公司,浙江温州325800

出　　处：《中国乳品工业》2021年第1期4-9,15,共7页China Dairy Industry

摘　　要：本课题以小鼠脾淋巴细胞为研究对象,对受到刺激前后细胞内多肽进行分离和鉴定。静息状态下淋巴细胞作为对照组,实验组用LPS刺激,均采用RPMI-1640不完全培养液养,破碎,收集胞内多肽,采用纳升液相-QExactive四极杆超高分辨轨道阱质谱仪(NanoLC-QExactivePlus)进行分析和鉴定,再通过网站搜索比对氨基酸序列确定是否为新发现多肽。结果表明,从静息状态下和受到刺激后小鼠脾淋巴细胞中共分离鉴定出385种多肽,肽链长度为8~30个氨基酸,其中139种为新发现多肽。In this study,mouse spleen lymphocytes were used as the research object to isolate and identify the intracellular polypeptides before and after stimulation.The lymphocytes in the resting state were served as the control group,and the experimental group were stimulated with LPS.All of them were cultured in RPMI-1640 incomplete culture medium,then broken,collected intracellular peptides,which were identified and analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer(Nano LC-Q Exactive Plus).Finally,the amino acid sequences were searched and compared through the websites to determine whether they were newly discovered.The results showed that 385 polypeptides were isolated and identified from mouse splenic lymphocytes in resting state and after stimulation,of which 139 were newly discovered peptides.The length of peptide chains were 8-30 amino acids.This study revealed the variety and quantity of bioactive peptides in spleen lymphocytes of mice before and after being stimulated by adverse external stimuli,laying a scientific foundation for the development of bioactive peptide health products and special medical formula foods.

关 键 词：淋巴细胞 多肽 分离鉴定 

分 类 号：Q516[生物学—生物化学]