斑点叉尾鮰鱼下脚料蛋白提取工艺的响应面法优化研究  

Optimization of Protein Extraction from Byproducts of Channel Catfish by Response Surface Methodology

作　　者：陈紫红 黄茂坤[2] CHEN Zi-hong;HUANG Mao-kun(College of Materials and Chemical Engineering,Liming Vocational University,Quanzhou 362000,China)

机构地区：[1]黎明职业大学材料与化学工程学院 [2]黎明职业大学,福建泉州362000

出　　处：《通化师范学院学报》2021年第2期70-75,共6页Journal of Tonghua Normal University

基　　金：福建省教育厅中青年教师教育科研项目(JZ180907);黎明职业大学校级一般课题(LZ2019115)。

摘　　要：采用冷冻干燥后的斑点叉尾鮰鱼下脚料为原料,以斑点叉尾鮰鱼下脚料的蛋白提取率为指标,考察液料比(w/v)、碱提温度及碱提pH对提取率的影响,在单因素实验的基础上,采用响应面法确定斑点叉尾鮰鱼下脚料蛋白的最优提取工艺.结果表明,提取斑点叉尾鮰鱼下脚料蛋白的最优条件为液料比1∶20、碱提pH 11.4、碱提温度38℃,在此优化工艺条件下斑点叉尾鮰鱼下脚料蛋白的实际提取率达82%±0.34%.Using byproducts of Channel catfish as research object,the effect of material/liquid ratio(w/v),the pH of alkaline extraction and the temperature of alkaline extraction on the extraction rate were investi⁃gated.The optimal conditions were found as follows:the ratio of material to liquid(w/v)was 1∶20,alkali extraction pH 11.4 and the temperature was 38℃.Under this conditions,the extraction rate of protein ex⁃traction from byproducts of Channel catfish was 82%±0.34%.

关 键 词：斑点叉尾鮰鱼 下脚料 蛋白质 响应面法 提取工艺 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]