ATF2在宫颈癌组织中的表达及沉默ATF2抑制自噬对宫颈癌细胞化疗敏感性的影响  

Expression of ATF2 in Cervical Cancer Tissues and the Effect of Silencing ATF2 to Inhibit Autophagy on Chemotherapy Sensitivity of Cervical Cancer Cells

作　　者：周展 李燚 ZHOU Zhan;LI Yi(Shandong Provincial Third Hospital,Shandong Jinan 250031,China)

机构地区：[1]山东省立第三医院妇科,山东济南250031

出　　处：《河北医学》2021年第2期207-211,共5页Hebei Medicine

基　　金：山东省医药卫生科研项目,(编号:2018WS283)。

摘　　要：目的:探讨ATF2在宫颈癌组织中的表达及沉默ATF2抑制自噬对宫颈癌细胞化疗敏感性的影响。方法:免疫组织化学法检测ATF2在宫颈癌组织中的表达;qRT-PCR和Western blot检测ATF2在宫颈癌组织和宫颈癌细胞系中的表达;细胞克隆形成实验检测HeLa细胞的增殖能力;Western blot检测HeLa细胞中LC3Ⅱ/LC3 I蛋白表达水平比例;细胞免疫荧光染色检测HeLa细胞中自噬体数目;细胞流式术检测HeLa细胞的凋亡率;Western blot检测HeLa细胞中Bax、Bcl-2蛋白的表达。结果:ATF2在宫颈癌组织和宫颈癌细胞系中高表达(P<0.01);沉默ATF2联合DDP使HeLa细胞的增殖能力下降(P<0.001)、LC3Ⅱ/LC3 I蛋白表达水平比例下降(P<0.01)和自噬体数目减少(P<0.01);沉默ATF2联合DDP使HeLa细胞的凋亡率增加(P<0.01),Bax蛋白表达水平上调(P<0.001),Bcl-2蛋白表达水平下调(P<0.001)。结论:ATF2在宫颈癌组织和宫颈癌细胞中高表达,沉默ATF2抑制自噬并增强HeLa对DDP的敏感性增加凋亡率,从而增强HeLa细胞的化疗敏感性。Objective:To investigate the expression of ATF2 in cervical cancer tissues and the effect of silencing ATF2 on the sensitivity of cervical cancer cells to the inhibition of autophagy.Methods:Immunohistochemical method was used to detect the expression of ATF2 in cervical cancer tissues;qRT-PCR and Western blot were used to detect the expression of ATF2 in cervical cancer tissues and cervical cancer cell lines;Cell clone formation experiment was used to detect the proliferation ability of HeLa cells;Western blot was used to analyze of the ratio of LC3 II/LC3 I protein expression levels in HeLa cells;Cellular immunofluorescence staining was used to detect the number of autophagosomes in HeLa cells;Cell flow cytometry was used to detect the apoptosis rate of HeLa cells;Western blot was used to detecte the expression of Bax and Bcl-2 protein in HeLa cells.Results:ATF2 was highly expressed in cervical cancer tissues and cervical cancer cell lines(P<0.01);Silent ATF2 combined with DDP reduced the proliferation capacity of HeLa cells(P<0.001),the ratio of LC3 II/LC3 I protein expression level(P<0.01)and the number of autophagosomes(P<0.01);Silent ATF2 combined with DDP increased the apoptosis rate of HeLa cells(P<0.01),Bax protein expression level was up-regulated(P<0.001),Bcl-2 protein expression level was down-regulated(P<0.001).Conclusion:Atf2 is highly expressed in cervical cancer tissues and cervical cancer cells.Silencing Atf2 inhibits autophagy,enhances the sensitivity of HeLa to DDP,and increases the apoptosis rate,thus enhancing the chemosensitivity of HeLa cells.

关 键 词：宫颈癌 ATF2 自噬 化疗敏感性 

分 类 号：R737.33[医药卫生—肿瘤]